Hotspot

trnL-UAG-ndhF

SSC · 124790-126366 · 1577 bp

Back to Campsis

Pi 0.456

MarkerSeek score 80.50

Primer available yes

Label trnL-UAG-ndhF

Feature Metadata

label_name: trnL-UAG-ndhF
feature_type: igs
region: SSC
length_bp: 1577
variable_sites: 609
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhF_p1	TTTAGGAGATGACATGCCGC	TGAAGTGGAAATTGTGCCGA	50.0	45.0	58.0	58.0	2411 bp	0.5	59.4
trnL-UAG-ndhF_p2	TGATCCACATAAAAGGGCGG	TGAAGTGGAAATTGTGCCGA	50.0	45.0	57.9	58.0	2942 bp	0.5	59.4
trnL-UAG-ndhF_p3	TTTAGGAGATGACATGCCGC	GCCCACATACGACGAAGATT	50.0	50.0	58.0	58.1	1793 bp	0.5	59.1
trnL-UAG-ndhF_p4	GAAGTAGCAAAGCCTTGGGT	TGAAGTGGAAATTGTGCCGA	50.0	45.0	58.1	58.0	2824 bp	0.5	59.1
trnL-UAG-ndhF_p5	TGATCCACATAAAAGGGCGG	GCCCACATACGACGAAGATT	50.0	50.0	57.9	58.1	2324 bp	0.5	59.1