Hotspot

ndhE-psaC

SSC · 121273-121522 · 250 bp

Back to Campsis

Pi 0.476

MarkerSeek score 76.80

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 250
variable_sites: 111
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	AACTATTGGCCCGGCTATTG	TACATCCGTCCCAGGGTATC	50.0	55.0	58.0	58.0	438 bp	0.5	59.8
ndhE-psaC_p2	CAACTATTGGCCCGGCTATT	TACATCCGTCCCAGGGTATC	50.0	55.0	58.0	58.0	439 bp	0.5	59.8
ndhE-psaC_p3	CTATTGGCCCGGCTATTGTT	TACATCCGTCCCAGGGTATC	50.0	55.0	58.0	58.0	436 bp	0.5	59.8
ndhE-psaC_p4	ACGTCCTGGTATGGGATCAT	TACATCCGTCCCAGGGTATC	50.0	55.0	57.9	58.0	1055 bp	0.5	59.1
ndhE-psaC_p5	CTATTGGCCCGGCTATTGTT	GACCCATACTGCGAGTTGTT	50.0	50.0	58.0	57.9	568 bp	0.5	59.1