Hotspot

ndhI-ndhG

SSC · 119871-120219 · 349 bp

Back to Campsis

Pi 0.48

MarkerSeek score 76.20

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 349
variable_sites: 146
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CTCCCGGTTGTTGATTGGAA	CGCAGCAGCTACAAAATACG	50.0	50.0	58.1	57.9	808 bp	0.5	58.6
ndhI-ndhG_p2	ACAAACAATACGAGCTGCCA	CGCAGCAGCTACAAAATACG	45.0	50.0	58.1	57.9	1001 bp	0.5	58.5
ndhI-ndhG_p3	CTCCCGGTTGTTGATTGGAA	CCCAACGGTCCAAAGATGAA	50.0	50.0	58.1	58.1	913 bp	0.5	58.5
ndhI-ndhG_p4	CTCCCGGTTGTTGATTGGAA	ATGATCCCATACCAGGACGT	50.0	50.0	58.1	57.9	996 bp	0.5	58.4
ndhI-ndhG_p5	ACAAACAATACGAGCTGCCA	CCCAACGGTCCAAAGATGAA	45.0	50.0	58.1	58.1	1106 bp	0.5	58.4