Hotspot

petB-petD

LSC · 77290-77482 · 193 bp

Back to Campsis

Pi 0.0688

MarkerSeek score 85.40

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 13
pi: 0.0688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	GCCGCTTCTTACTGCTGTAT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.1	57.7	290 bp	1.0	81.6
petB-petD_p2	GCCGCTTCTTACTGCTGTAT	GTTACACGGTGGTTGGAACA	50.0	50.0	58.1	58.3	371 bp	1.0	80.5
petB-petD_p3	GCCGCTTCTTACTGCTGTAT	ATAGGGGTTACACGGTGGTT	50.0	50.0	58.1	58.3	377 bp	1.0	80.4
petB-petD_p4	GCCGCTTCTTACTGCTGTAT	AGTTCAAGTCACACACTCCC	50.0	50.0	58.1	57.4	291 bp	1.0	79.2
petB-petD_p5	GCCGCTTCTTACTGCTGTAT	TATAGGGGTTACACGGTGGT	50.0	50.0	58.1	57.5	378 bp	1.0	78.7