primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46770	47506	797-798	1.000000	82.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46770	47506	829-830	1.000000	81.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAAATAGGAAAAGGCCGGCT	CCGTAGCGTCTACCGATTTC	20	20	45.0	55.0	57.8	58.2	28.6	10.2	0.480165	46770	47506	852-853	1.000000	81.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46770	47506	791-792	1.000000	81.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	46770	47506	829-830	1.000000	80.3
petB-petD_p1	petB-petD	1	GCCGCTTCTTACTGCTGTAT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.1	57.7	4.1	0.0	0.384258	77290	77482	290-290	1.000000	81.6
petB-petD_p2	petB-petD	2	GCCGCTTCTTACTGCTGTAT	GTTACACGGTGGTTGGAACA	20	20	50.0	50.0	58.1	58.3	4.1	13.5	0.392621	77290	77482	371-371	1.000000	80.5
petB-petD_p3	petB-petD	3	GCCGCTTCTTACTGCTGTAT	ATAGGGGTTACACGGTGGTT	20	20	50.0	50.0	58.1	58.3	4.1	13.5	0.398433	77290	77482	377-377	1.000000	80.4
petB-petD_p4	petB-petD	4	GCCGCTTCTTACTGCTGTAT	AGTTCAAGTCACACACTCCC	20	20	50.0	50.0	58.1	57.4	4.1	0.0	0.680739	77290	77482	291-291	1.000000	79.2
petB-petD_p5	petB-petD	5	GCCGCTTCTTACTGCTGTAT	TATAGGGGTTACACGGTGGT	20	20	50.0	50.0	58.1	57.5	4.1	13.5	0.607139	77290	77482	378-378	1.000000	78.7
ycf1_p1	ycf1	1	AGGAAGCACCAAACCAATCA	ACATACTGAAACGACTGCCA	20	20	45.0	45.0	57.6	57.2	3.0	0.0	1.211749	114940	115059	415-415	0.500000	50.3
ycf1_p2	ycf1	2	AAGAGGAAGCACCAAACCAA	ACATACTGAAACGACTGCCA	20	20	45.0	45.0	57.5	57.2	3.0	0.0	1.284285	114940	115059	418-418	0.500000	49.7
ycf1_p3	ycf1	3	CATTCGTCTGTCTGTCCCAA	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	57.5	57.2	0.0	0.0	1.287359	114940	115059	488-488	0.500000	49.7
ycf1_p4	ycf1	4	AACCCATTCGTCTGTCTGTC	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	57.5	57.2	0.0	0.0	1.289051	114940	115059	492-492	0.500000	49.7
ycf1_p5	ycf1	5	GAACCCATTCGTCTGTCTGT	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	57.5	57.2	0.0	0.0	1.289051	114940	115059	493-493	0.500000	49.7
ycf1-rps15_p1	ycf1-rps15	1	AGCAGCCAAGTTTCACATGA	CAGCAGTCGTTGACGTTTTC	20	20	45.0	50.0	58.0	58.0	0.0	3.2	0.019715	115158	115549	788-788	0.500000	59.8
ycf1-rps15_p2	ycf1-rps15	2	AGCAGCCAAGTTTCACATGA	TCCCAACGCGTTACATAAGG	20	20	45.0	50.0	58.0	58.0	0.0	26.1	0.027464	115158	115549	1177-1177	0.500000	59.8
ycf1-rps15_p3	ycf1-rps15	3	AGCAGCCAAGTTTCACATGA	AGTCGTTGACGTTTTCCCAA	20	20	45.0	45.0	58.0	58.0	0.0	3.2	0.041530	115158	115549	784-784	0.500000	59.7
ycf1-rps15_p4	ycf1-rps15	4	AGCAGCCAAGTTTCACATGA	CCGAGTCATTCTTCCATGCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.042947	115158	115549	956-956	0.500000	59.7
ycf1-rps15_p5	ycf1-rps15	5	GCAGCCAAGTTTCACATGAC	CAGCAGTCGTTGACGTTTTC	20	20	50.0	50.0	57.9	58.0	0.0	3.2	0.061283	115158	115549	787-787	0.500000	59.5
rps15_p1	rps15	1	AGCAGCCAAGTTTCACATGA	TCCCAACGCGTTACATAAGG	20	20	45.0	50.0	58.0	58.0	0.0	26.1	0.027464	115550	115822	1177-1177	0.500000	59.8
rps15_p2	rps15	2	AGCAGCCAAGTTTCACATGA	CCGAGTCATTCTTCCATGCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.042947	115550	115822	956-956	0.500000	59.7
rps15_p3	rps15	3	GCAGCCAAGTTTCACATGAC	TCCCAACGCGTTACATAAGG	20	20	50.0	50.0	57.9	58.0	0.0	26.1	0.069031	115550	115822	1176-1176	0.500000	59.4
rps15_p4	rps15	4	AGCAGCCAAGTTTCACATGA	AACACCATGCATTGATGGGT	20	20	45.0	45.0	58.0	58.1	0.0	8.9	0.073150	115550	115822	1040-1040	0.500000	59.4
rps15_p5	rps15	5	CAGCAGCCAAGTTTCACATG	TCCCAACGCGTTACATAAGG	20	20	50.0	50.0	57.9	58.0	0.0	26.1	0.073263	115550	115822	1178-1178	0.500000	59.4
ndhH_p1	ndhH	1	GAAAACGTCAACGACTGCTG	TGAAGAATTCCCAAAGGCCC	20	20	50.0	50.0	58.0	58.1	9.9	0.0	0.067997	115919	117100	1601-1601	0.500000	59.5
ndhH_p2	ndhH	2	GAAAACGTCAACGACTGCTG	GCTATGGAGGGACCGATACT	20	20	50.0	55.0	58.0	58.1	9.9	0.0	0.079080	115919	117100	1694-1694	0.500000	59.4
ndhH_p3	ndhH	3	GAAAACGTCAACGACTGCTG	CTGCTATGGAGGGACCGATA	20	20	50.0	55.0	58.0	58.1	9.9	0.0	0.080457	115919	117100	1696-1696	0.500000	59.4
ndhH_p4	ndhH	4	TTGGGAAAACGTCAACGACT	TGAAGAATTCCCAAAGGCCC	20	20	45.0	50.0	58.0	58.1	9.9	0.0	0.089812	115919	117100	1605-1605	0.500000	59.3
ndhH_p5	ndhH	5	TGCATGGAAGAATGACTCGG	TGAAGAATTCCCAAAGGCCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.091229	115919	117100	1433-1433	0.500000	59.3
ndhA_p1	ndhA	1	CAGGCTCTGGAAGGAATTCC	TTCCAATCAACAACCGGGAG	20	20	55.0	50.0	58.0	58.1	17.3	6.3	0.145601	117102	119284	2868-2868	0.500000	58.8
ndhA_p2	ndhA	2	CAGGCTCTGGAAGGAATTCC	TGGCAGCTCGTATTGTTTGT	20	20	55.0	45.0	58.0	58.1	17.3	0.0	0.159012	117102	119284	2675-2675	0.500000	58.7
ndhA_p3	ndhA	3	CAGGCTCTGGAAGGAATTCC	CCAATCAACAACCGGGAGAT	20	20	55.0	50.0	58.0	57.9	17.3	6.3	0.178659	117102	119284	2866-2866	0.500000	58.6
ndhA_p4	ndhA	4	AGAGTCGAAGCACCAAAAGG	TTCCAATCAACAACCGGGAG	20	20	50.0	50.0	58.1	58.1	0.0	6.3	0.211478	117102	119284	2712-2712	0.500000	58.3
ndhA_p5	ndhA	5	AGTCGAAGCACCAAAAGGAG	TTCCAATCAACAACCGGGAG	20	20	50.0	50.0	58.1	58.1	0.0	6.3	0.211478	117102	119284	2710-2710	0.500000	58.3
ndhI_p1	ndhI	1	CTCTAGCCGAGTGTGAGAGA	TGAAAGGGTGGTGTGGATTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037302	119364	119870	1255-1255	0.500000	59.7
ndhI_p2	ndhI	2	CTCTAGCCGAGTGTGAGAGA	TGAATGAAAGGGTGGTGTGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037302	119364	119870	1259-1259	0.500000	59.7
ndhI_p3	ndhI	3	TCTCTAGCCGAGTGTGAGAG	TGAAAGGGTGGTGTGGATTG	20	20	55.0	50.0	58.0	58.0	1.1	0.0	0.037302	119364	119870	1256-1256	0.500000	59.7
ndhI_p4	ndhI	4	TCTCTAGCCGAGTGTGAGAG	TGAATGAAAGGGTGGTGTGG	20	20	55.0	50.0	58.0	58.0	1.1	0.0	0.037302	119364	119870	1260-1260	0.500000	59.7
ndhI_p5	ndhI	5	TGTTCCGGAGCTTTTTGACA	TGAAAGGGTGGTGTGGATTG	20	20	45.0	50.0	58.0	58.0	19.7	0.0	0.051605	119364	119870	1059-1059	0.500000	59.6
ndhI-ndhG_p1	ndhI-ndhG	1	CTCCCGGTTGTTGATTGGAA	CGCAGCAGCTACAAAATACG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.172868	119871	120219	808-808	0.500000	58.6
ndhI-ndhG_p2	ndhI-ndhG	2	ACAAACAATACGAGCTGCCA	CGCAGCAGCTACAAAATACG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.186279	119871	120219	1001-1001	0.500000	58.5
ndhI-ndhG_p3	ndhI-ndhG	3	CTCCCGGTTGTTGATTGGAA	CCCAACGGTCCAAAGATGAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.191426	119871	120219	913-913	0.500000	58.5
ndhI-ndhG_p4	ndhI-ndhG	4	CTCCCGGTTGTTGATTGGAA	ATGATCCCATACCAGGACGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.198867	119871	120219	996-996	0.500000	58.4
ndhI-ndhG_p5	ndhI-ndhG	5	ACAAACAATACGAGCTGCCA	CCCAACGGTCCAAAGATGAA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.204837	119871	120219	1106-1106	0.500000	58.4
ndhG_p1	ndhG	1	CAATCCACACCACCCTTTCA	AATAGCCGGGCCAATAGTTG	20	20	50.0	50.0	58.0	58.0	0.0	17.0	0.024788	120220	120750	1115-1115	0.500000	59.8
ndhG_p2	ndhG	2	CAATCCACACCACCCTTTCA	CAATAGCCGGGCCAATAGTT	20	20	50.0	50.0	58.0	58.0	0.0	17.0	0.024788	120220	120750	1116-1116	0.500000	59.8
ndhG_p3	ndhG	3	CCACACCACCCTTTCATTCA	AACAATAGCCGGGCCAATAG	20	20	50.0	50.0	58.0	58.0	0.0	17.0	0.024788	120220	120750	1114-1114	0.500000	59.8
ndhG_p4	ndhG	4	CCACACCACCCTTTCATTCA	AATAGCCGGGCCAATAGTTG	20	20	50.0	50.0	58.0	58.0	0.0	17.0	0.024788	120220	120750	1111-1111	0.500000	59.8
ndhG_p5	ndhG	5	CCACACCACCCTTTCATTCA	CAATAGCCGGGCCAATAGTT	20	20	50.0	50.0	58.0	58.0	0.0	17.0	0.024788	120220	120750	1112-1112	0.500000	59.8
ndhE-psaC_p1	ndhE-psaC	1	AACTATTGGCCCGGCTATTG	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	58.0	58.0	9.9	5.0	0.024114	121273	121522	438-438	0.500000	59.8
ndhE-psaC_p2	ndhE-psaC	2	CAACTATTGGCCCGGCTATT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	58.0	58.0	9.9	5.0	0.024114	121273	121522	439-439	0.500000	59.8
ndhE-psaC_p3	ndhE-psaC	3	CTATTGGCCCGGCTATTGTT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	58.0	58.0	9.9	5.0	0.024114	121273	121522	436-436	0.500000	59.8
ndhE-psaC_p4	ndhE-psaC	4	ACGTCCTGGTATGGGATCAT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	57.9	58.0	0.0	5.0	0.108524	121273	121522	1055-1055	0.500000	59.1
ndhE-psaC_p5	ndhE-psaC	5	CTATTGGCCCGGCTATTGTT	GACCCATACTGCGAGTTGTT	20	20	50.0	50.0	58.0	57.9	9.9	0.0	0.113506	121273	121522	568-568	0.500000	59.1
ndhD_p1	ndhD	1	GATACCCTGGGACGGATGTA	TTTGTGTGAACTGGCGAGAA	20	20	55.0	45.0	58.0	58.0	22.2	1.4	0.036629	121880	123400	1979-1979	0.500000	59.7
ndhD_p2	ndhD	2	AACTATTGGCCCGGCTATTG	TTTGTGTGAACTGGCGAGAA	20	20	50.0	45.0	58.0	58.0	9.9	1.4	0.050002	121880	123400	2397-2397	0.500000	59.6
ndhD_p3	ndhD	3	CAACTATTGGCCCGGCTATT	TTTGTGTGAACTGGCGAGAA	20	20	50.0	45.0	58.0	58.0	9.9	1.4	0.050002	121880	123400	2398-2398	0.500000	59.6
ndhD_p4	ndhD	4	CTATTGGCCCGGCTATTGTT	TTTGTGTGAACTGGCGAGAA	20	20	50.0	45.0	58.0	58.0	9.9	1.4	0.050002	121880	123400	2395-2395	0.500000	59.6
ndhD_p5	ndhD	5	GATACCCTGGGACGGATGTA	TTGGAGTTGGGACCCAAAAG	20	20	55.0	50.0	58.0	57.9	22.2	27.9	0.086024	121880	123400	2257-2257	0.500000	59.3
ndhD-ccsA_p1	ndhD-ccsA	1	TCAGTAGCTTTTCGATGGCC	CCGCCCTTTTATGTGGATCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.075510	123401	123648	1155-1155	0.500000	59.4
ndhD-ccsA_p2	ndhD-ccsA	2	CAGTAGCTTTTCGATGGCCT	CCGCCCTTTTATGTGGATCA	20	20	50.0	50.0	58.0	57.9	3.3	0.0	0.081173	123401	123648	1154-1154	0.500000	59.4
ndhD-ccsA_p3	ndhD-ccsA	3	TTGGTTGATGATGGTAGGCG	CCGCCCTTTTATGTGGATCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.088292	123401	123648	1454-1454	0.500000	59.3
ndhD-ccsA_p4	ndhD-ccsA	4	TCAGTAGCTTTTCGATGGCC	TTGGAGTTGGGACCCAAAAG	20	20	50.0	50.0	58.0	57.9	0.0	27.9	0.104830	123401	123648	833-833	0.500000	59.2
ndhD-ccsA_p5	ndhD-ccsA	5	CAGTAGCTTTTCGATGGCCT	TTGGAGTTGGGACCCAAAAG	20	20	50.0	50.0	58.0	57.9	3.3	27.9	0.110492	123401	123648	832-832	0.500000	59.1
ccsA_p1	ccsA	1	TCAGTAGCTTTTCGATGGCC	GCGGCATGTCATCTCCTAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.070617	123649	124626	1686-1686	0.500000	59.4
ccsA_p2	ccsA	2	CAGTAGCTTTTCGATGGCCT	GCGGCATGTCATCTCCTAAA	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.076279	123649	124626	1685-1685	0.500000	59.4
ccsA_p3	ccsA	3	TTCTCGCCAGTTCACACAAA	GCGGCATGTCATCTCCTAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.077699	123649	124626	1151-1151	0.500000	59.4
ccsA_p4	ccsA	4	TCAGTAGCTTTTCGATGGCC	GAGTTCAAGACAAGCCGCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.104462	123649	124626	1773-1773	0.500000	59.2
ccsA_p5	ccsA	5	CAGTAGCTTTTCGATGGCCT	GAGTTCAAGACAAGCCGCTA	20	20	50.0	50.0	58.0	57.9	3.3	0.0	0.110124	123649	124626	1772-1772	0.500000	59.1
trnL-UAG-ndhF_p1	trnL-UAG-ndhF	1	TTTAGGAGATGACATGCCGC	TGAAGTGGAAATTGTGCCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.072176	124790	126366	2411-2411	0.500000	59.4
trnL-UAG-ndhF_p2	trnL-UAG-ndhF	2	TGATCCACATAAAAGGGCGG	TGAAGTGGAAATTGTGCCGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.077069	124790	126366	2942-2942	0.500000	59.4
trnL-UAG-ndhF_p3	trnL-UAG-ndhF	3	TTTAGGAGATGACATGCCGC	GCCCACATACGACGAAGATT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110509	124790	126366	1793-1793	0.500000	59.1
trnL-UAG-ndhF_p4	trnL-UAG-ndhF	4	GAAGTAGCAAAGCCTTGGGT	TGAAGTGGAAATTGTGCCGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.114678	124790	126366	2824-2824	0.500000	59.1
trnL-UAG-ndhF_p5	trnL-UAG-ndhF	5	TGATCCACATAAAAGGGCGG	GCCCACATACGACGAAGATT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.115402	124790	126366	2324-2324	0.500000	59.1
ndhF_p1	ndhF	1	AAAGAGGTAAGGTCCGGTGA	ATGCCGTCACCCATTCTTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058194	126367	128604	2672-2672	0.500000	59.5
ndhF_p2	ndhF	2	AAGAGGTAAGGTCCGGTGAA	ATGCCGTCACCCATTCTTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058194	126367	128604	2671-2671	0.500000	59.5
ndhF_p3	ndhF	3	AAAGAGGTAAGGTCCGGTGA	AAGTGAGGAAGAAAGCGTGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.135572	126367	128604	2610-2610	0.500000	58.9
ndhF_p4	ndhF	4	AAGAGGTAAGGTCCGGTGAA	AAGTGAGGAAGAAAGCGTGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.135572	126367	128604	2609-2609	0.500000	58.9
ndhF_p5	ndhF	5	AAGAGGTAAGGTCCGGTGAA	GTGAGGAAGAAAGCGTGGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.140854	126367	128604	2607-2607	0.500000	58.9