Hotspot

rbcL

LSC · 58211-59638 · 1428 bp

Back to Camphora

Pi 0.007

MarkerSeek score 62.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 10
pi: 0.007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	ACAATTAGGGGAACGAACGG	50.0	50.0	57.3	57.9	1479 bp	1.0	74.0
rbcL_p2	TCATGAGTTGTAGGGAGGGA	ACAATTAGGGGAACGAACGG	50.0	50.0	57.1	57.9	1482 bp	1.0	72.2
rbcL_p3	CATGAGTTGTAGGGAGGGAC	ACAATTAGGGGAACGAACGG	55.0	50.0	57.0	57.9	1481 bp	1.0	71.8
rbcL_p4	TGAGTTGTAGGGAGGGACTT	GGGAACGAACGGGAATTACT	50.0	50.0	57.3	57.6	1471 bp	1.0	71.7
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	ACAATTAGGGGAACGAACGG	47.6	50.0	57.8	57.9	1483 bp	1.0	70.1