Hotspot

psbT

LSC · 77653-77769 · 117 bp

Back to Camphora

Pi 0.0256

MarkerSeek score 67.20

Primer available yes

Label psbT

Feature Metadata

label_name: psbT
feature_type: gene
region: LSC
length_bp: 117
variable_sites: 3
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT_p1	TCGCCTTTCTTTGCTCTTGT	TCGAGGAACACGGAGACTAA	45.0	50.0	58.0	57.8	278 bp	1.0	79.1
psbT_p2	TCGCCTTTCTTTGCTCTTGT	TTCGAGGAACACGGAGACTA	45.0	50.0	58.0	57.8	279 bp	1.0	79.1
psbT_p3	TCGCCTTTCTTTGCTCTTGT	CATTCGAGGAACACGGAGAC	45.0	55.0	58.0	58.4	281 bp	1.0	77.6
psbT_p4	TCGCCTTTCTTTGCTCTTGT	GATCCATTCGAGGAACACGG	45.0	55.0	58.0	58.4	285 bp	1.0	77.2
psbT_p5	TCGCCTTTCTTTGCTCTTGT	ATTCGAGGAACACGGAGACT	45.0	50.0	58.0	58.5	280 bp	1.0	77.0