Hotspot

rpl2-rpl23

LSC · 89271-89303 · 33 bp

Back to Camphora

Pi 0.0357

MarkerSeek score 67.30

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 33
variable_sites: 1
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TGGATCCTTTGCGTATGCTC	CGCTTCAACCGGGTTATTCT	50.0	50.0	58.0	58.3	139-144 bp	1.0	80.9
rpl2-rpl23_p2	GCTCGGGGTAGAAGTTTTGT	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	123-128 bp	1.0	80.3
rpl2-rpl23_p3	TGGATCCTTTGCGTATGCTC	ATCATTACGCTTCAACCGGG	50.0	50.0	58.0	58.3	146-151 bp	1.0	80.2
rpl2-rpl23_p4	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	124-129 bp	1.0	80.2
rpl2-rpl23_p5	TGCTCGGGGTAGAAGTTTTG	ATCATTACGCTTCAACCGGG	50.0	50.0	57.8	58.3	131-136 bp	1.0	79.5