Hotspot

rps19-rpl2

LSC · 87694-87772 · 79 bp

Back to Camphora

Pi 0.0135

MarkerSeek score 64.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	GTTATCCTGCGCTTGGAAGA	55.0	50.0	59.2	58.0	261-266 bp	1.0	72.3
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CCTGCGCTTGGAAGAAAAAG	55.0	50.0	59.2	57.9	256-261 bp	1.0	72.1
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	ATCCTGCGCTTGGAAGAAAA	55.0	45.0	59.2	58.1	258-263 bp	1.0	71.8
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	275-280 bp	1.0	71.2
rps19-rpl2_p5	GCCCGGGACCAAGTTACTAT	GGCCCCGATTGGTAGAAAAA	55.0	50.0	59.2	58.2	299-304 bp	1.0	71.0