Hotspot

trnP(TGG)-psaJ

LSC · 70293-70667 · 375 bp

Back to Camphora

Pi 0.0187
MarkerSeek score 64.80
Primer available yes
Label trnP(TGG)-psaJ

Feature Metadata

label_name
trnP(TGG)-psaJ
feature_type
igs
region
LSC
length_bp
375
variable_sites
7
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP(TGG)-psaJ_p1 ACCCGTGACATTTTGTACCC AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 546 bp 1.0 78.6
trnP(TGG)-psaJ_p2 CCCGTGACATTTTGTACCCA AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 545 bp 1.0 78.6
trnP(TGG)-psaJ_p3 ACAGGATTTGAACCCGTGAC AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 557 bp 1.0 78.6
trnP(TGG)-psaJ_p4 CAGGATTTGAACCCGTGACA AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 556 bp 1.0 78.5
trnP(TGG)-psaJ_p5 TGACAGGATTTGAACCCGTG AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 559 bp 1.0 78.5