Hotspot

petA-psbJ

LSC · 66313-67440 · 1128 bp

Back to Camphora

Pi 0.0241

MarkerSeek score 73.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1128
variable_sites: 27
pi: 0.0241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	45.0	55.0	58.2	58.2	1273-1309 bp	1.0	79.3
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	45.0	55.0	58.2	58.6	1274-1310 bp	1.0	76.3
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	45.0	50.0	58.2	57.1	1272-1308 bp	1.0	73.9
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1266-1302 bp	1.0	73.7
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	45.0	55.0	57.1	58.2	1272-1308 bp	1.0	73.3