Hotspot

ycf3-trnS(GGA)

LSC · 46697-47504 · 808 bp

Back to Camphora

Pi 0.00743

MarkerSeek score 63.00

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 6
pi: 0.00743
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	TCTCCTGAGGTTGTCGGAAT	TTGTGGTCGGTTGGTGAATT	50.0	45.0	58.1	57.9	1021-1023 bp	1.0	79.5
ycf3-trnS(GGA)_p2	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	991-993 bp	1.0	78.8
ycf3-trnS(GGA)_p3	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	941-943 bp	1.0	78.5
ycf3-trnS(GGA)_p4	TCTCCTGAGGTTGTCGGAAT	ATTGTGGTCGGTTGGTGAAT	50.0	45.0	58.1	57.7	1022-1024 bp	1.0	77.7
ycf3-trnS(GGA)_p5	TTCTCCTGAGGTTGTCGGAA	TTGTGGTCGGTTGGTGAATT	50.0	45.0	58.3	57.9	1022-1024 bp	1.0	77.7