Hotspot

trnS(TGA)-psbZ

LSC · 37924-38246 · 323 bp

Back to Camphora

Pi 0.00312
MarkerSeek score 52.20
Primer available yes
Label trnS(TGA)-psbZ

Feature Metadata

label_name
trnS(TGA)-psbZ
feature_type
igs
region
LSC
length_bp
323
variable_sites
1
pi
0.00312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(TGA)-psbZ_p1 CAACCACTCAGCCATCTCTC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.0 57.0 442-444 bp 1.0 72.7
trnS(TGA)-psbZ_p2 CAACCACTCAGCCATCTCTC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.0 57.0 375-377 bp 1.0 72.5
trnS(TGA)-psbZ_p3 GAGCTATCAACCACTCAGCC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 449-451 bp 1.0 72.4
trnS(TGA)-psbZ_p4 GAGCTATCAACCACTCAGCC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 382-384 bp 1.0 72.2
trnS(TGA)-psbZ_p5 CGGTTTTCAAGACCAGAGCT ACCAACCATCAGAAGAAGCA 50.0 45.0 58.1 57.0 464-466 bp 1.0 71.9