Hotspot

psbC-trnS(TGA)

LSC · 37616-37830 · 215 bp

Back to Camphora

Pi 0.0421

MarkerSeek score 73.40

Primer available yes

Label psbC-trnS(TGA)

Feature Metadata

label_name: psbC-trnS(TGA)
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 9
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS(TGA)_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	336-337 bp	1.0	80.9
psbC-trnS(TGA)_p2	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.2	58.1	329-330 bp	1.0	80.7
psbC-trnS(TGA)_p3	CCTGTTCTTTCCATGACCCC	AGCTCTGGTCTTGAAAACCG	55.0	50.0	58.2	58.1	314-315 bp	1.0	80.3
psbC-trnS(TGA)_p4	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	267-268 bp	1.0	80.0
psbC-trnS(TGA)_p5	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	379-380 bp	1.0	78.9