Hotspot

trnS(GCT)-trnG(TCC)

LSC · 9493-10310 · 818 bp

Back to Camphora

Pi 0.00733

MarkerSeek score 63.00

Primer available yes

Label trnS(GCT)-trnG(TCC)

Feature Metadata

label_name: trnS(GCT)-trnG(TCC)
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 6
pi: 0.00733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCT)-trnG(TCC)_p1	ATTAGCAATCCGCCGCTTTA	AGGAATCAAACGAAAGATCCCT	45.0	40.9	58.0	57.4	937 bp	1.0	59.6
trnS(GCT)-trnG(TCC)_p2	GCTTTAGTCCACTCAGCCAT	AGGAATCAAACGAAAGATCCCT	50.0	40.9	57.6	57.4	923 bp	1.0	56.6
trnS(GCT)-trnG(TCC)_p3	AGAAGAAACGGAGAGAGGGG	AGGAATCAAACGAAAGATCCCT	55.0	40.9	58.4	57.4	993 bp	1.0	56.4
trnS(GCT)-trnG(TCC)_p4	AGTCCACTCAGCCATCTCTC	AGGAATCAAACGAAAGATCCCT	55.0	40.9	58.5	57.4	918 bp	1.0	55.8
trnS(GCT)-trnG(TCC)_p5	ATTAGCAATCCGCCGCTTTA	GGAATCAAACGAAAGATCCCT	45.0	42.9	58.0	55.9	936 bp	1.0	55.7