Hotspot

trnH(GTG)-psbA

LSC · 97-494 · 398 bp

Back to Camphora

Pi 0.0203
MarkerSeek score 52.60
Primer available yes
Label trnH(GTG)-psbA

Feature Metadata

label_name
trnH(GTG)-psbA
feature_type
igs
region
LSC
length_bp
398
variable_sites
8
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GTG)-psbA_p1 GATCCACTTGGCTACATCCG TCAGTGCTATGCATGGTTCC 55.0 50.0 58.1 58.0 847-851 bp 1.0 79.9
trnH(GTG)-psbA_p2 CAATCCACTGCCTTGATCCA TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 861-865 bp 1.0 79.9
trnH(GTG)-psbA_p3 GATCCACTTGGCTACATCCG CAGTGCTATGCATGGTTCCT 55.0 50.0 58.1 58.0 846-850 bp 1.0 79.9
trnH(GTG)-psbA_p4 AATCCACTGCCTTGATCCAC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 860-864 bp 1.0 79.9
trnH(GTG)-psbA_p5 ACAATCCACTGCCTTGATCC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 862-866 bp 1.0 79.9