Hotspot

psbE-petL

LSC · 62484-63653 · 1170 bp

Back to Campeiostachys

Pi 0.00228

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 4
pi: 0.00228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	45.0	50.0	58.0	58.3	1372-1374 bp	1.0	78.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	50.0	50.0	57.9	58.3	1361-1363 bp	1.0	77.9
psbE-petL_p3	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1326-1328 bp	1.0	76.4
psbE-petL_p4	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1324-1326 bp	1.0	76.4
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	50.0	50.0	58.2	58.3	1363-1365 bp	1.0	76.4