Hotspot

ycf3-trnS-GGA

LSC · 43860-44474 · 615 bp

Back to Campeiostachys

Pi 0.00108

MarkerSeek score 64.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 1
pi: 0.00108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	718-798 bp	1.0	82.4
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	668-748 bp	1.0	82.1
ycf3-trnS-GGA_p3	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	719-799 bp	1.0	80.6
ycf3-trnS-GGA_p4	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	720-800 bp	1.0	80.6
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	669-749 bp	1.0	80.3