primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGGGTATTTCTGTCTTTTCTTTCT	AATACGTGTGCTTGGGAGTC	24	20	33.3	50.0	56.2	57.9	0.0	0.0	5.881121	93	1154	1167-1167	1.000000	40.1
psbA_p2	psbA	2	TGGGTATTTCTGTCTTTTCTTTCTT	AATACGTGTGCTTGGGAGTC	25	20	32.0	50.0	56.9	57.9	0.0	0.0	6.229422	93	1154	1167-1167	1.000000	40.1
psbA_p3	psbA	3	TTGGGTATTTCTGTCTTTTCTTTCT	AATACGTGTGCTTGGGAGTC	25	20	32.0	50.0	56.9	57.9	0.0	0.0	6.229422	93	1154	1168-1168	1.000000	40.1
psbA_p4	psbA	4	GGGTATTTCTGTCTTTTCTTTCTTCA	AATACGTGTGCTTGGGAGTC	26	20	34.6	50.0	57.8	57.9	0.0	0.0	6.261184	93	1154	1166-1166	1.000000	40.1
psbA_p5	psbA	5	TGGGTATTTCTGTCTTTTCTTTCTTC	AATACGTGTGCTTGGGAGTC	26	20	34.6	50.0	57.8	57.9	0.0	0.0	6.261184	93	1154	1167-1167	1.000000	40.1
trnK-UUU_p1	trnK-UUU	1	CAACCAAGTCAGCCCAAAGA	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.345360	1376	3950	2752-2752	1.000000	77.3
trnK-UUU_p2	trnK-UUU	2	TCAACCAAGTCAGCCCAAAG	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.345360	1376	3950	2753-2753	1.000000	77.3
trnK-UUU_p3	trnK-UUU	3	AATGTCAACCAAGTCAGCCC	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.4	58.0	0.0	0.0	0.415734	1376	3950	2757-2757	1.000000	76.8
trnK-UUU_p4	trnK-UUU	4	GTCAACCAAGTCAGCCCAAA	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.6	58.0	0.0	0.0	0.637864	1376	3950	2754-2754	1.000000	75.0
trnK-UUU_p5	trnK-UUU	5	ACCAAGTCAGCCCAAAGATT	AATGTTTTGACAGAAGCGCG	20	20	45.0	45.0	57.3	58.0	0.0	0.0	0.719767	1376	3950	2750-2750	1.000000	74.3
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	21	20	47.6	45.0	58.3	58.0	0.0	0.0	1.340971	3224	3912	876-876	1.000000	69.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTGAAAGAGAAGTGGG	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	56.5	58.0	0.0	0.0	1.570959	3224	3912	876-876	1.000000	67.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	CTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	56.5	58.0	0.0	0.0	1.570959	3224	3912	875-875	1.000000	67.6
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCCTGAAAGAGAAGTGGGTAGA	AATGTTTTGACAGAAGCGCG	22	20	45.5	45.0	57.9	58.0	0.0	0.0	2.164474	3224	3912	874-874	1.000000	62.9
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTGAAAGAGAAGTGGGTA	AATGTTTTGACAGAAGCGCG	22	20	45.5	45.0	58.1	58.0	0.0	0.0	2.178422	3224	3912	876-876	1.000000	62.8
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	20	20	45.0	55.0	58.0	58.1	0.0	5.0	0.148662	7630	8609	1132-1134	1.000000	79.2
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	7630	8609	1110-1112	1.000000	78.1
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	20	20	55.0	55.0	57.8	58.1	0.0	5.0	0.343212	7630	8609	1183-1185	1.000000	77.6
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.349331	7630	8609	1094-1096	1.000000	77.6
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	20	20	45.0	50.0	58.0	58.3	0.0	5.0	0.369381	7630	8609	1125-1127	1.000000	77.4
trnfM-CAU-trnT-GGU_p1	trnfM-CAU-trnT-GGU	1	CCTCAAGGTTATGAGCCTCG	TGAACCGATGACTTATGCCT	20	20	55.0	45.0	57.8	56.6	0.0	0.0	1.602911	12683	14652	2065-2066	1.000000	67.3
trnfM-CAU-trnT-GGU_p2	trnfM-CAU-trnT-GGU	2	CCTCGTGAGCTACCAAACTG	TGAACCGATGACTTATGCCT	20	20	55.0	45.0	58.3	56.6	0.0	0.0	1.656902	12683	14652	2050-2051	1.000000	66.9
trnfM-CAU-trnT-GGU_p3	trnfM-CAU-trnT-GGU	3	CCTCAAGGTTATGAGCCTCG	TGACTTACGTCTTAGCACGAG	20	21	55.0	47.6	57.8	57.5	0.0	10.2	1.697927	12683	14652	2125-2127	1.000000	66.6
trnfM-CAU-trnT-GGU_p4	trnfM-CAU-trnT-GGU	4	CCTCAAGGTTATGAGCCTCG	CCGATGACTTATGCCTTACCA	20	21	55.0	47.6	57.8	57.5	0.0	0.0	1.700223	12683	14652	2061-2062	1.000000	66.6
trnfM-CAU-trnT-GGU_p5	trnfM-CAU-trnT-GGU	5	CCTCAAGGTTATGAGCCTCG	ACCGATGACTTATGCCTTACC	20	21	55.0	47.6	57.8	57.5	0.0	0.0	1.701859	12683	14652	2062-2063	1.000000	66.5
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	15470	15828	480-482	1.000000	76.3
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	15470	15828	447-449	1.000000	73.9
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	15470	15828	446-448	1.000000	73.7
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	20	20	50.0	50.0	58.0	57.0	0.0	7.1	1.041608	15470	15828	479-481	1.000000	72.2
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GATGCCCGAGTGGTTAATGG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.7	58.5	0.0	7.1	1.168334	15470	15828	465-467	1.000000	71.2
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	18189	19346	1226-1227	1.000000	79.4
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	18189	19346	1233-1234	1.000000	78.3
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18189	19346	1241-1242	1.000000	77.2
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18189	19346	1240-1241	1.000000	77.2
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	20	20	50.0	45.0	58.1	57.6	14.6	13.0	0.442790	18189	19346	1305-1306	1.000000	76.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.212764	43860	44474	718-798	1.000000	82.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.1	57.8	0.0	5.6	0.254079	43860	44474	668-748	1.000000	82.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.439292	43860	44474	719-799	1.000000	80.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.439292	43860	44474	720-800	1.000000	80.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.3	57.8	0.0	5.6	0.480608	43860	44474	669-749	1.000000	80.3
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	ATGTAGGAGAGATGGCCGAG	AGATAGGAAGTGGGTCGGTC	20	20	55.0	55.0	58.4	58.2	0.0	0.0	0.595373	44562	44867	462-468	1.000000	76.4
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	ATGTAGGAGAGATGGCCGAG	ATAGGAAGTGGGTCGGTCTC	20	20	55.0	55.0	58.4	58.2	0.0	0.0	0.595373	44562	44867	460-466	1.000000	76.4
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	ATGTAGGAGAGATGGCCGAG	GATAGGAAGTGGGTCGGTCT	20	20	55.0	55.0	58.4	58.2	0.0	0.0	0.595373	44562	44867	461-467	1.000000	76.4
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	ATGTAGGAGAGATGGCCGAG	GAAGTGGGTCGGTCTCAAAA	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.623157	44562	44867	456-462	1.000000	76.2
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	TTCGAATCCCTCTCTTTCCG	ATAGGAAGTGGGTCGGTCTC	20	20	50.0	55.0	57.0	58.2	0.0	0.0	1.183586	44562	44867	388-394	1.000000	72.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	45847	46462	716-717	1.000000	80.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	45847	46462	708-709	1.000000	79.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	45847	46462	686-687	1.000000	79.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	45847	46462	706-707	1.000000	78.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	45847	46462	715-716	1.000000	77.8
rbcL-psaI_p1	rbcL-psaI	1	CCGAAATCTTTGGGGACGAT	AACTCGCTATACCTGGCTCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.113115	55891	57532	2159-2165	1.000000	79.7
rbcL-psaI_p2	rbcL-psaI	2	TCTTCACATTCACCGTGCAA	AACTCGCTATACCTGGCTCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.114312	55891	57532	2461-2467	1.000000	79.7
rbcL-psaI_p3	rbcL-psaI	3	TTCTTCACATTCACCGTGCA	AACTCGCTATACCTGGCTCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.114312	55891	57532	2462-2468	1.000000	79.7
rbcL-psaI_p4	rbcL-psaI	4	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.0	57.9	0.0	26.9	0.115800	55891	57532	1973-1974	1.000000	79.4
rbcL-psaI_p5	rbcL-psaI	5	TCTTCACATTCACCGTGCAA	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	58.0	57.9	0.0	26.9	0.116996	55891	57532	2275-2276	1.000000	79.4
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	20	20	45.0	50.0	58.0	58.3	0.0	7.6	0.278273	62484	63653	1372-1374	1.000000	78.1
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	57.9	58.3	6.4	7.6	0.312268	62484	63653	1361-1363	1.000000	77.9
psbE-petL_p3	psbE-petL	3	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	62484	63653	1326-1328	1.000000	76.4
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	62484	63653	1324-1326	1.000000	76.4
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	58.2	58.3	0.0	7.6	0.498226	62484	63653	1363-1365	1.000000	76.4
ndhF-rpl32_p1	ndhF-rpl32	1	GAAGAGGGAATTGTGCGGAT	GATGCTCATTACCGCTGGAA	20	20	50.0	50.0	58.0	58.0	0.0	1.5	0.090564	103802	104716	1788-1793	1.000000	79.9
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	GATGCTCATTACCGCTGGAA	20	20	55.0	50.0	58.1	58.0	0.0	1.5	0.160019	103802	104716	1099-1104	1.000000	79.7
ndhF-rpl32_p3	ndhF-rpl32	3	CATTCCCACGAGTTCCCAAA	GATGCTCATTACCGCTGGAA	20	20	50.0	50.0	58.1	58.0	0.0	1.5	0.142154	103802	104716	1517-1522	1.000000	79.5
ndhF-rpl32_p4	ndhF-rpl32	4	GAAGAGGGAATTGTGCGGAT	ACCCTTTGGTTTTGGATGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.145762	103802	104716	1802-1807	1.000000	79.4
ndhF-rpl32_p5	ndhF-rpl32	5	AACACATTCCCACGAGTTCC	GATGCTCATTACCGCTGGAA	20	20	50.0	50.0	58.1	58.0	0.0	1.5	0.154232	103802	104716	1521-1526	1.000000	79.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050149	104909	105610	840-841	1.000000	80.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105116	104909	105610	843-844	1.000000	79.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	104909	105610	812-813	1.000000	79.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	104909	105610	811-812	1.000000	79.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.170718	104909	105610	813-814	1.000000	79.3
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	TCTGGACCACGAGAACTCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006910	105762	106730	1374-1374	1.000000	80.3
ccsA_p2	ccsA	2	CTCAAGCATCTCGGTTCGAA	TTCTGGACCACGAGAACTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006910	105762	106730	1375-1375	1.000000	80.3
ccsA_p3	ccsA	3	CTCAAGCATCTCGGTTCGAA	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.015248	105762	106730	1378-1378	1.000000	80.2
ccsA_p4	ccsA	4	CTCAAGCATCTCGGTTCGAA	TGAGTGGTTTTGTCGCAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034967	105762	106730	1571-1571	1.000000	80.1
ccsA_p5	ccsA	5	CTCAAGCATCTCGGTTCGAA	GCCTTACCAGGAATGAGTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.047831	105762	106730	1584-1584	1.000000	80.0
ndhH_p1	ndhH	1	AGGAGGGTCAAAATGGGTAGA	TTTGATGAGCCTCGCTTTGA	21	20	47.6	45.0	58.1	57.8	0.0	0.0	1.254177	113310	114491	1364-1364	1.000000	70.1
ndhH_p2	ndhH	2	AAAGGAGGGTCAAAATGGGT	TTTGATGAGCCTCGCTTTGA	20	20	45.0	45.0	56.9	57.8	0.0	0.0	1.277647	113310	114491	1366-1366	1.000000	70.0
ndhH_p3	ndhH	3	TCCCAAAAGGAGGGTCAAAA	TTTGATGAGCCTCGCTTTGA	20	20	45.0	45.0	56.8	57.8	0.0	0.0	1.340108	113310	114491	1371-1371	1.000000	69.5
ndhH_p4	ndhH	4	AGGAGGGTCAAAATGGGTAGA	TTGATGAGCCTCGCTTTGAG	21	20	47.6	50.0	58.1	58.3	0.0	0.0	1.340198	113310	114491	1363-1363	1.000000	69.5
ndhH_p5	ndhH	5	AAAGGAGGGTCAAAATGGGT	TTGATGAGCCTCGCTTTGAG	20	20	45.0	50.0	56.9	58.3	0.0	0.0	1.363668	113310	114491	1365-1365	1.000000	69.3
trnI-GAU_p1	trnI-GAU	1	GTTCCGGGGAATCCAGTTAC	AACTCCAAAAACCCGTCCTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.057048	121614	122491	2179-2179	1.000000	79.7
trnI-GAU_p2	trnI-GAU	2	TCTATTCTTTTCCCTGGCGC	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061331	121614	122491	1474-1474	1.000000	79.7
trnI-GAU_p3	trnI-GAU	3	GCCAAGTGGAGTATGCATGA	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	10.2	0.0	0.065630	121614	122491	1511-1511	1.000000	79.6
trnI-GAU_p4	trnI-GAU	4	AGCCAAGTGGAGTATGCATG	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	10.2	0.0	0.071388	121614	122491	1512-1512	1.000000	79.6
trnI-GAU_p5	trnI-GAU	5	TAGATCTGACCAACTGCCCA	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	5.2	0.0	0.071734	121614	122491	1750-1750	1.000000	79.6