Hotspot

ndhH-rps12

IRa · 144940-155079 · 10140 bp

Back to Campanula

Pi 0.0653

MarkerSeek score 70.40

Primer available yes

Label ndhH-rps12

Feature Metadata

label_name: ndhH-rps12
feature_type: igs
region: IRa
length_bp: 10140
variable_sites: 1469
pi: 0.0653
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps12_p1	CCCAAGGTGTTTAGCAAGGT	ATTGAAAAACAGACCGCCCT	50.0	45.0	58.0	58.0	1368-1399 bp	1.0	90.3
ndhH-rps12_p2	GTCCCCAAGGTGTTTAGCAA	ATTGAAAAACAGACCGCCCT	50.0	45.0	58.0	58.0	1371-1402 bp	1.0	90.2
ndhH-rps12_p3	CCCAAGGTGTTTAGCAAGGT	GCAAGAACGGACTAAGAGGG	50.0	55.0	58.0	58.0	1345-1376 bp	1.0	90.2
ndhH-rps12_p4	TAAGCCAGCACTTTGAAGCA	TTTGACCCTAAGCACCCAAC	45.0	50.0	58.0	58.0	2075 bp	0.6	63.8
ndhH-rps12_p5	TGCCCTTATGATTTCAGGCC	TTTGACCCTAAGCACCCAAC	50.0	50.0	57.9	58.0	2726 bp	0.6	63.3