Hotspot

rps12-ndhH

IRb · 116586-126725 · 10140 bp

Back to Campanula

Pi 0.0658

MarkerSeek score 66.70

Primer available yes

Label rps12-ndhH

Feature Metadata

label_name: rps12-ndhH
feature_type: igs
region: IRb
length_bp: 10140
variable_sites: 1480
pi: 0.0658
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ndhH_p1	TTTGACCCTAAGCACCCAAC	TAAGCCAGCACTTTGAAGCA	50.0	45.0	58.0	58.0	2075 bp	0.6	63.8
rps12-ndhH_p2	TTTGACCCTAAGCACCCAAC	TGCCCTTATGATTTCAGGCC	50.0	50.0	58.0	57.9	2726 bp	0.6	63.3
rps12-ndhH_p3	CTGAAGTAACCCATGCCACA	TCATAAACAGCTAACAAGTCCTC	50.0	39.1	57.8	56.3	644 bp	0.6	24.8
rps12-ndhH_p4	CTGAAGTAACCCATGCCACA	TCATAAACAGCTAACAAGTCCT	50.0	36.4	57.8	55.1	644 bp	0.6	24.0
rps12-ndhH_p5	CTGAAGTAACCCATGCCACA	CATAAACAGCTAACAAGTCCTC	50.0	40.9	57.8	54.9	643 bp	0.6	24.0