Hotspot

trnM-CAU-trnL-CAA

LSC · 94404-102495 · 8092 bp

Back to Campanula

Pi 0.0671

MarkerSeek score 71.60

Primer available yes

Label trnM-CAU-trnL-CAA

Feature Metadata

label_name: trnM-CAU-trnL-CAA
feature_type: igs
region: LSC
length_bp: 8092
variable_sites: 1274
pi: 0.0671
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnL-CAA_p1	AAAGCGCGATTCTTGAGGAT	CCTCCCCCGTTTGTACAAAA	45.0	50.0	58.0	58.0	1677-1707 bp	0.8	76.3
trnM-CAU-trnL-CAA_p2	AAAGCGCGATTCTTGAGGAT	AAGAGGTCCTTCATCAGGCT	45.0	50.0	58.0	58.0	1216-1246 bp	0.8	76.3
trnM-CAU-trnL-CAA_p3	AAAGCGCGATTCTTGAGGAT	TGAAGATCAGCGAACCATGG	45.0	50.0	58.0	58.0	865-1057 bp	0.8	72.7
trnM-CAU-trnL-CAA_p4	AAAGCGCGATTCTTGAGGAT	AAGATCAGCGAACCATGGAC	45.0	50.0	58.0	58.0	863-1055 bp	0.8	72.6
trnM-CAU-trnL-CAA_p5	CTGGGACGATCAAGAATGCA	CCTCCCCCGTTTGTACAAAA	50.0	50.0	58.0	58.0	2070 bp	0.6	64.7