Hotspot

trnM-CAU-trnM-CAU

LSC · 44635-44863 · 229 bp

Back to Campanula

Pi 0.0623

MarkerSeek score 74.00

Primer available yes

Label trnM-CAU-trnM-CAU

Feature Metadata

label_name: trnM-CAU-trnM-CAU
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 31
pi: 0.0623
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnM-CAU_p1	TATTGCTTTCATACGGCGGG	TGGCGAATTCGTAGGTTCAA	50.0	45.0	58.4	57.5	307-319 bp	1.0	82.5
trnM-CAU-trnM-CAU_p2	TATTGCTTTCATACGGCGGG	TTGGCGAATTCGTAGGTTCA	50.0	45.0	58.4	57.5	308-320 bp	1.0	82.5
trnM-CAU-trnM-CAU_p3	TATTGCTTTCATACGGCGGG	GCACCCAACTCATAATTGGC	50.0	50.0	58.4	57.4	323-335 bp	1.0	81.0
trnM-CAU-trnM-CAU_p4	TATTGCTTTCATACGGCGGG	GCTGAATGGTTAAAGCACCC	50.0	50.0	58.4	57.3	337-349 bp	1.0	80.1
trnM-CAU-trnM-CAU_p5	TATTGCTTTCATACGGCGGG	GGCTGAATGGTTAAAGCACC	50.0	50.0	58.4	57.3	338-350 bp	1.0	80.1