Pi
0.0411
MarkerSeek score
69.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 800-818 bp | 1.0 | 87.5 |
| trnT-UGU-trnL-UAA_p2 | GACTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 831-849 bp | 1.0 | 86.9 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 808-826 bp | 1.0 | 86.8 |
| trnT-UGU-trnL-UAA_p4 | GACTCGAACCGATGACCATC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 839-857 bp | 1.0 | 86.3 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 814-832 bp | 1.0 | 85.4 |