Hotspot

trnV-GAC-rpl20

LSC · 29486-31538 · 2053 bp

Back to Campanula

Pi 0.189

MarkerSeek score 75.30

Primer available yes

Label trnV-GAC-rpl20

Feature Metadata

label_name: trnV-GAC-rpl20
feature_type: igs
region: LSC
length_bp: 2053
variable_sites: 793
pi: 0.189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rpl20_p1	CTTCGGGGATTCGTTCAGAG	TATCCCCGGCTAATTCTCGT	55.0	50.0	58.1	58.0	2527-2808 bp	0.8	83.4
trnV-GAC-rpl20_p2	GATTCGTTCAGAGCAAGGGT	TATCCCCGGCTAATTCTCGT	50.0	50.0	57.9	58.0	2520-2801 bp	0.8	83.2
trnV-GAC-rpl20_p3	TACGCAATGGGAAGGTTGAG	TATCCCCGGCTAATTCTCGT	50.0	50.0	57.9	58.0	2402-2683 bp	0.8	83.1
trnV-GAC-rpl20_p4	TGGTTCATCATTCCAAGGGC	TATCCCCGGCTAATTCTCGT	50.0	50.0	58.1	58.0	2363-2644 bp	0.8	82.8
trnV-GAC-rpl20_p5	GTTCATCATTCCAAGGGCCT	TATCCCCGGCTAATTCTCGT	50.0	50.0	57.9	58.0	2361-2642 bp	0.8	82.8