primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGCCTTCCAAGCTAACGATG	TTGGGTCTGCAGCTCAAATT	20	20	50.0	45.0	58.0	58.0	0.0	17.5	0.030190	10128	10240	799-805	1.000000	85.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	CCTAGCCTTCCAAGCTAACG	TTGGGTCTGCAGCTCAAATT	20	20	55.0	45.0	58.1	58.0	0.0	17.5	0.059027	10128	10240	802-808	1.000000	84.7
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AGCCTTCCAAGCTAACGATG	TTGGATTTGGCGCAATTGAC	20	20	50.0	45.0	58.0	57.9	0.0	10.8	0.107251	10128	10240	746-752	1.000000	84.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	CCTAGCCTTCCAAGCTAACG	TTGGATTTGGCGCAATTGAC	20	20	55.0	45.0	58.1	57.9	0.0	10.8	0.136088	10128	10240	749-755	1.000000	84.3
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AAATGCTGACTTGACGTCGA	TTGGGTCTGCAGCTCAAATT	20	20	45.0	45.0	57.9	58.0	11.6	17.5	0.149267	10128	10240	830-836	1.000000	83.8
atpF-atpH_p1	atpF-atpH	1	GTACAGCTGGTTGGATCCTG	GGGTCGGAACAAAGAAAGGT	20	20	55.0	50.0	58.0	58.0	19.9	0.0	0.052782	13120	13540	1092-1092	0.200000	47.6
atpF-atpH_p2	atpF-atpH	2	GTACAGCTGGTTGGATCCTG	TGGGTCGGAACAAAGAAAGG	20	20	55.0	50.0	58.0	58.0	19.9	0.0	0.054179	13120	13540	1093-1093	0.200000	47.6
atpF-atpH_p3	atpF-atpH	3	AGTACAGCTGGTTGGATCCT	GGGTCGGAACAAAGAAAGGT	20	20	50.0	50.0	58.0	58.0	19.9	0.0	0.064656	13120	13540	1093-1093	0.200000	47.5
atpF-atpH_p4	atpF-atpH	4	AGTACAGCTGGTTGGATCCT	TGGGTCGGAACAAAGAAAGG	20	20	50.0	50.0	58.0	58.0	19.9	0.0	0.066052	13120	13540	1094-1094	0.200000	47.5
atpF-atpH_p5	atpF-atpH	5	GTACAGCTGGTTGGATCCTG	ATTTCTGCCGCTTCCGTTAT	20	20	55.0	45.0	58.0	58.0	19.9	0.0	0.068225	13120	13540	847-847	0.200000	47.5
rpl2-rps19_p1	rpl2-rps19	1	TGGGTAGAGCCGGATCTAAG	TCTTCAGGCTAGTACCCTCG	20	20	55.0	55.0	58.0	57.9	29.9	0.0	0.057434	16642	16700	881-885	1.000000	83.0
rpl2-rps19_p2	rpl2-rps19	2	TGGGTAGAGCCGGATCTAAG	CTAGAAGGTGGCGAGCATTT	20	20	55.0	50.0	58.0	58.0	29.9	0.0	0.033040	16642	16700	818-818	0.200000	47.7
rpl2-rps19_p3	rpl2-rps19	3	GATGACGGAAGGGAAAGACC	CTAGAAGGTGGCGAGCATTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.055427	16642	16700	1257-1257	0.200000	47.6
rpl2-rps19_p4	rpl2-rps19	4	CCAGATGACGGAAGGGAAAG	CTAGAAGGTGGCGAGCATTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.059677	16642	16700	1260-1260	0.200000	47.5
rpl2-rps19_p5	rpl2-rps19	5	GATGACGGAAGGGAAAGACC	TCTTCAGGCTAGTACCCTCG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.079821	16642	16700	1320-1320	0.200000	47.4
psbB-trnV-GAC_p1	psbB-trnV-GAC	1	ACACCCATATTCCAGGACCA	ACCTTGTCTATTTGGCGTCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129615	28668	29413	1868-1868	0.600000	63.3
psbB-trnV-GAC_p2	psbB-trnV-GAC	2	TGCACCAAAACCAAAGCAAG	ACCTTGTCTATTTGGCGTCC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.130780	28668	29413	1828-1828	0.600000	63.3
psbB-trnV-GAC_p3	psbB-trnV-GAC	3	CCCCGCGAGAAATAAATGGA	ACCTTGTCTATTTGGCGTCC	20	20	50.0	50.0	58.0	57.9	8.2	0.0	0.147631	28668	29413	1804-1804	0.600000	63.2
psbB-trnV-GAC_p4	psbB-trnV-GAC	4	ACACCCATATTCCAGGACCA	CCCCCAAATCCGTTTACCAT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.173224	28668	29413	1808-1808	0.600000	63.0
psbB-trnV-GAC_p5	psbB-trnV-GAC	5	CCAACCACCCCATGAATTGA	ACCTTGTCTATTTGGCGTCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.173625	28668	29413	1597-1597	0.600000	62.9
trnV-GAC-rpl20_p1	trnV-GAC-rpl20	1	CTTCGGGGATTCGTTCAGAG	TATCCCCGGCTAATTCTCGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.077475	29486	31538	2527-2808	0.800000	83.4
trnV-GAC-rpl20_p2	trnV-GAC-rpl20	2	GATTCGTTCAGAGCAAGGGT	TATCCCCGGCTAATTCTCGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.105975	29486	31538	2520-2801	0.800000	83.2
trnV-GAC-rpl20_p3	trnV-GAC-rpl20	3	TACGCAATGGGAAGGTTGAG	TATCCCCGGCTAATTCTCGT	20	20	50.0	50.0	57.9	58.0	3.8	0.0	0.113320	29486	31538	2402-2683	0.800000	83.1
trnV-GAC-rpl20_p4	trnV-GAC-rpl20	4	TGGTTCATCATTCCAAGGGC	TATCCCCGGCTAATTCTCGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.154066	29486	31538	2363-2644	0.800000	82.8
trnV-GAC-rpl20_p5	trnV-GAC-rpl20	5	GTTCATCATTCCAAGGGCCT	TATCCCCGGCTAATTCTCGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.155343	29486	31538	2361-2642	0.800000	82.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	38846	39595	800-818	1.000000	87.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GACTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	38846	39595	831-849	1.000000	86.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	38846	39595	808-826	1.000000	86.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GACTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	38846	39595	839-857	1.000000	86.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	38846	39595	814-832	1.000000	85.4
trnV-UAC-trnM-CAU_p1	trnV-UAC-trnM-CAU	1	TGTGCATTGGGCTCTTTCAT	GATCTGAAAGTCGATCCGGG	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.214048	44366	44561	1434-1442	1.000000	84.3
trnV-UAC-trnM-CAU_p2	trnV-UAC-trnM-CAU	2	TTTTTGAGGCCCTTCGACTC	TCTAAAAACCATTGCGGGCT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.189918	44366	44561	1086-1094	0.600000	69.3
trnV-UAC-trnM-CAU_p3	trnV-UAC-trnM-CAU	3	TGTGCATTGGGCTCTTTCAT	TCTAAAAACCATTGCGGGCT	20	20	45.0	45.0	58.1	58.1	0.0	0.0	0.154641	44366	44561	1355-1363	0.600000	68.8
trnV-UAC-trnM-CAU_p4	trnV-UAC-trnM-CAU	4	TGTGCATTGGGCTCTTTCAT	AAACCATTGCGGGCTTTAGA	20	20	45.0	45.0	58.1	58.1	0.0	0.0	0.154641	44366	44561	1358-1358	0.200000	46.8
trnV-UAC-trnM-CAU_p5	trnV-UAC-trnM-CAU	5	TTTTTGAGGCCCTTCGACTC	AAACCATTGCGGGCTTTAGA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.189918	44366	44561	1089-1089	0.200000	46.5
trnM-CAU-trnM-CAU_p1	trnM-CAU-trnM-CAU	1	TATTGCTTTCATACGGCGGG	TGGCGAATTCGTAGGTTCAA	20	20	50.0	45.0	58.4	57.5	13.2	27.2	0.870386	44635	44863	307-319	1.000000	82.5
trnM-CAU-trnM-CAU_p2	trnM-CAU-trnM-CAU	2	TATTGCTTTCATACGGCGGG	TTGGCGAATTCGTAGGTTCA	20	20	50.0	45.0	58.4	57.5	13.2	27.2	0.870386	44635	44863	308-320	1.000000	82.5
trnM-CAU-trnM-CAU_p3	trnM-CAU-trnM-CAU	3	TATTGCTTTCATACGGCGGG	GCACCCAACTCATAATTGGC	20	20	50.0	50.0	58.4	57.4	13.2	0.0	0.995818	44635	44863	323-335	1.000000	81.0
trnM-CAU-trnM-CAU_p4	trnM-CAU-trnM-CAU	4	TATTGCTTTCATACGGCGGG	GCTGAATGGTTAAAGCACCC	20	20	50.0	50.0	58.4	57.3	13.2	0.0	1.066075	44635	44863	337-349	1.000000	80.1
trnM-CAU-trnM-CAU_p5	trnM-CAU-trnM-CAU	5	TATTGCTTTCATACGGCGGG	GGCTGAATGGTTAAAGCACC	20	20	50.0	50.0	58.4	57.3	13.2	0.0	1.066075	44635	44863	338-350	1.000000	80.1
trnE-UUC-trnY-GUA_p1	trnE-UUC-trnY-GUA	1	TCTCTTTCAAGGAGGCAACG	CCAGCGTAGACATATTGCCA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.408774	56290	56348	167-167	1.000000	80.1
trnE-UUC-trnY-GUA_p2	trnE-UUC-trnY-GUA	2	TCTCTTTCAAGGAGGCAACG	ACCAGCGTAGACATATTGCC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.410326	56290	56348	168-168	1.000000	80.1
trnE-UUC-trnY-GUA_p3	trnE-UUC-trnY-GUA	3	TCTCTTTCAAGGAGGCAACG	CGAATTTACAGTCCGTCCCC	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.521328	56290	56348	146-146	1.000000	79.7
trnE-UUC-trnY-GUA_p4	trnE-UUC-trnY-GUA	4	TCTCTTTCAAGGAGGCAACG	CCATCGCATGGTAGATTGGT	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.430676	56290	56348	214-214	1.000000	79.2
trnE-UUC-trnY-GUA_p5	trnE-UUC-trnY-GUA	5	TCGTCTAGTGGTTCAGGACA	CCAGCGTAGACATATTGCCA	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.520481	56290	56348	189-189	1.000000	78.8
trnD-GUC-psbJ_p1	trnD-GUC-psbJ	1	CATCCCCCATTCCCAGATTG	GCGAAAAAGAAAGGGCTTCC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.071363	56607	58294	2343-2640	0.800000	79.0
trnD-GUC-psbJ_p2	trnD-GUC-psbJ	2	CATCCCCCATTCCCAGATTG	TTCCTTCCCCTTTTTCGCAA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.080099	56607	58294	2327-2624	0.800000	78.9
trnD-GUC-psbJ_p3	trnD-GUC-psbJ	3	CATCCCCCATTCCCAGATTG	CTTTTTCGCAACGCTTCCTT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.125042	56607	58294	2318-2615	0.800000	78.6
trnD-GUC-psbJ_p4	trnD-GUC-psbJ	4	ATCCCCCATTCCCAGATTGA	GCGAAAAAGAAAGGGCTTCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.140369	56607	58294	2342-2639	0.800000	78.5
trnD-GUC-psbJ_p5	trnD-GUC-psbJ	5	TCCCCCATTCCCAGATTGAT	GCGAAAAAGAAAGGGCTTCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.140369	56607	58294	2341-2638	0.800000	78.5
ndhB-trnM-CAU_p1	ndhB-trnM-CAU	1	TTGCTCAGGATACCCTTTTT	CCTGTTAAGCATCCATGGCT	20	20	40.0	50.0	54.5	57.9	0.0	7.0	3.554923	93840	94329	595-1066	1.000000	62.3
ndhB-trnM-CAU_p2	ndhB-trnM-CAU	2	ATTGCTCAGGATACCCTTTT	CCTGTTAAGCATCCATGGCT	20	20	40.0	50.0	54.3	57.9	0.0	7.0	3.799408	93840	94329	596-1067	1.000000	60.4
ndhB-trnM-CAU_p3	ndhB-trnM-CAU	3	ATTGCTCAGGATACCCTTTTT	CCTGTTAAGCATCCATGGCT	21	20	38.1	50.0	55.1	57.9	0.0	7.0	3.926497	93840	94329	596-1067	1.000000	59.4
ndhB-trnM-CAU_p4	ndhB-trnM-CAU	4	TTGCTCAGGATACCCTTTTT	TGGCGAATTCGTAGGTTCAA	20	20	40.0	45.0	54.5	57.5	0.0	27.2	3.951013	93840	94329	548-1019	1.000000	59.4
ndhB-trnM-CAU_p5	ndhB-trnM-CAU	5	TTGCTCAGGATACCCTTTTT	TTGGCGAATTCGTAGGTTCA	20	20	40.0	45.0	54.5	57.5	0.0	27.2	3.951013	93840	94329	549-1020	1.000000	59.4
trnM-CAU-trnL-CAA_p1	trnM-CAU-trnL-CAA	1	AAAGCGCGATTCTTGAGGAT	CCTCCCCCGTTTGTACAAAA	20	20	45.0	50.0	58.0	58.0	11.2	22.1	0.045772	95700	95819	1677-1707	0.800000	76.3
trnM-CAU-trnL-CAA_p2	trnM-CAU-trnL-CAA	2	AAAGCGCGATTCTTGAGGAT	AAGAGGTCCTTCATCAGGCT	20	20	45.0	50.0	58.0	58.0	11.2	0.0	0.065853	95700	95819	1216-1246	0.800000	76.3
trnM-CAU-trnL-CAA_p3	trnM-CAU-trnL-CAA	3	AAAGCGCGATTCTTGAGGAT	TGAAGATCAGCGAACCATGG	20	20	45.0	50.0	58.0	58.0	11.2	11.2	0.058258	95700	95819	865-1057	0.800000	72.7
trnM-CAU-trnL-CAA_p4	trnM-CAU-trnL-CAA	4	AAAGCGCGATTCTTGAGGAT	AAGATCAGCGAACCATGGAC	20	20	45.0	50.0	58.0	58.0	11.2	21.5	0.059677	95700	95819	863-1055	0.800000	72.6
trnM-CAU-trnL-CAA_p5	trnM-CAU-trnL-CAA	5	CTGGGACGATCAAGAATGCA	CCTCCCCCGTTTGTACAAAA	20	20	50.0	50.0	58.0	58.0	0.0	22.1	0.044354	95700	95819	2070-2070	0.600000	64.7
rps12-ndhH_p1	rps12-ndhH	1	TTTGACCCTAAGCACCCAAC	TAAGCCAGCACTTTGAAGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030439	124541	124660	2075-2075	0.600000	63.8
rps12-ndhH_p2	rps12-ndhH	2	TTTGACCCTAAGCACCCAAC	TGCCCTTATGATTTCAGGCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.082071	124541	124660	2726-2726	0.600000	63.3
rps12-ndhH_p3	rps12-ndhH	3	CTGAAGTAACCCATGCCACA	TCATAAACAGCTAACAAGTCCTC	20	23	50.0	39.1	57.8	56.3	0.0	0.0	4.905706	124396	124995	644-644	0.600000	24.8
rps12-ndhH_p4	rps12-ndhH	4	CTGAAGTAACCCATGCCACA	TCATAAACAGCTAACAAGTCCT	20	22	50.0	36.4	57.8	55.1	0.0	0.0	5.089411	124396	124995	644-644	0.600000	24.0
rps12-ndhH_p5	rps12-ndhH	5	CTGAAGTAACCCATGCCACA	CATAAACAGCTAACAAGTCCTC	20	22	50.0	40.9	57.8	54.9	0.0	0.0	5.341536	124396	124995	643-643	0.600000	24.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GTAGGCCAATTCTTACCGCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.040511	135437	136019	1130-1147	0.800000	75.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGTAGGCCAATTCTTACCGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.040511	135437	136019	1131-1148	0.800000	75.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GTAGGCCAATTCTTACCGCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.041881	135437	136019	1131-1148	0.800000	75.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTAGGCCAATTCTTACCGC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.041881	135437	136019	1132-1149	0.800000	75.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GTAGGCCAATTCTTACCGCA	CACAGAAGAGCCGCATAACT	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.048416	135437	136019	1748-1750	0.600000	63.6
ccsA-ndhD_p1	ccsA-ndhD	1	GGGTATTCTTTCGGGAGCAG	AACGAAATGGGTGGAGTAGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.154498	137151	137423	967-1014	1.000000	86.4
ccsA-ndhD_p2	ccsA-ndhD	2	ACCCAAGGCTTTGCTACTTC	AACGAAATGGGTGGAGTAGC	20	20	50.0	50.0	58.1	57.9	2.4	0.0	0.198367	137151	137423	1335-1382	1.000000	85.1
ccsA-ndhD_p3	ccsA-ndhD	3	AGTTATGCGGCTCTTCTGTG	AACGAAATGGGTGGAGTAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.125161	137151	137423	1224-1246	0.800000	77.0
ccsA-ndhD_p4	ccsA-ndhD	4	AGTTATGCGGCTCTTCTGTG	CCTTTTCGATGGCTTCCCTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.176312	137151	137423	1169-1191	0.800000	76.6
ccsA-ndhD_p5	ccsA-ndhD	5	AAATTCTGCAATTGTGGCCG	AACGAAATGGGTGGAGTAGC	20	20	45.0	50.0	57.9	57.9	7.4	0.0	0.192591	137151	137423	823-870	0.800000	73.0
ndhH-rps12_p1	ndhH-rps12	1	CCCAAGGTGTTTAGCAAGGT	ATTGAAAAACAGACCGCCCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.009247	154223	154472	1368-1399	1.000000	90.3
ndhH-rps12_p2	ndhH-rps12	2	GTCCCCAAGGTGTTTAGCAA	ATTGAAAAACAGACCGCCCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.014917	154223	154472	1371-1402	1.000000	90.2
ndhH-rps12_p3	ndhH-rps12	3	CCCAAGGTGTTTAGCAAGGT	GCAAGAACGGACTAAGAGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.016164	154223	154472	1345-1376	1.000000	90.2
ndhH-rps12_p4	ndhH-rps12	4	TAAGCCAGCACTTTGAAGCA	TTTGACCCTAAGCACCCAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.030439	146997	147116	2075-2075	0.600000	63.8
ndhH-rps12_p5	ndhH-rps12	5	TGCCCTTATGATTTCAGGCC	TTTGACCCTAAGCACCCAAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082071	146997	147116	2726-2726	0.600000	63.3