Hotspot

rpoC2

LSC · 61412-65548 · 4137 bp

Back to Camellia

Pi 0.00125

MarkerSeek score 56.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4137
variable_sites: 124
pi: 0.00125
unique_haplotype_count: 90
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATGTTACTGCATGGATCGGG	ATTGTTCTCGAATGGGTCCA	50.0	45.0	57.7	56.8	277-283 bp	0.932203	71.8
rpoC2_p2	ATGTTACTGCATGGATCGGG	TTGTTCTCGAATGGGTCCAT	50.0	45.0	57.7	56.8	276-282 bp	0.932203	71.8
rpoC2_p3	ATGTTACTGCATGGATCGGG	AATTGTTCTCGAATGGGTCCA	50.0	42.9	57.7	57.6	278-284 bp	0.932203	69.7
rpoC2_p4	ATGTTACTGCATGGATCGGG	TCCAATTGTTCTCGAATGGGT	50.0	42.9	57.7	57.6	281-287 bp	0.932203	69.7
rpoC2_p5	GTTACTGCATGGATCGGGAT	TTGTTCTCGAATGGGTCCAT	50.0	45.0	57.4	56.8	274-280 bp	0.932203	69.5