Hotspot

psbA

LSC · 44790-45851 · 1062 bp

Back to Camellia

Pi 0.00149

MarkerSeek score 56.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 67
pi: 0.00149
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGGAACAATAACGCCTTCCT	AAATTCGTGTGCTTGGGAGT	45.0	45.0	56.8	58.0	1199 bp	0.991525	76.2
psbA_p2	GGAACAATAACGCCTTCCTG	AAATTCGTGTGCTTGGGAGT	50.0	45.0	56.8	58.0	1198 bp	0.991525	75.9
psbA_p3	AGGAACAATAACGCCTTCCTG	AAATTCGTGTGCTTGGGAGT	47.6	45.0	58.3	58.0	1199 bp	0.991525	75.3
psbA_p4	GGAACAATAACGCCTTCCTGA	AAATTCGTGTGCTTGGGAGT	47.6	45.0	58.3	58.0	1198 bp	0.991525	75.2
psbA_p5	ACGCCTTCCTGATAGAACAAG	AAATTCGTGTGCTTGGGAGT	47.6	45.0	57.7	58.0	1189 bp	0.983051	75.2