Hotspot

psbE-petL

LSC · 110989-112280 · 1292 bp

Back to Camellia

Pi 0.00168

MarkerSeek score 58.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 60
pi: 0.00168
unique_haplotype_count: 53
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1468-1501 bp	1.0	79.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1457-1490 bp	1.0	79.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1380-1413 bp	1.0	78.7
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1375-1408 bp	1.0	78.4
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1459-1492 bp	1.0	78.0