Hotspot

trnT-UGU-trnL-UAA

LSC · 92592-93592 · 1001 bp

Back to Camellia

Pi 0.00226

MarkerSeek score 60.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 46
pi: 0.00226
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	693-1131 bp	0.983051	84.7
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	701-1139 bp	0.983051	84.2
trnT-UGU-trnL-UAA_p3	AAATGCGATGCTCTAACCCC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.3	58.0	665-1103 bp	0.983051	84.2
trnT-UGU-trnL-UAA_p4	AAATGCGATGCTCTAACCCC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.3	58.1	673-1111 bp	0.983051	83.6
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	707-1145 bp	0.983051	82.8