Hotspot

ycf3-trnS-GGA

LSC · 90324-91200 · 877 bp

Back to Camellia

Pi 0.00159
MarkerSeek score 57.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
877
variable_sites
43
pi
0.00159
unique_haplotype_count
47
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 944-994 bp 1.0 78.1
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 894-944 bp 1.0 78.0
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTG 47.6 50.0 58.0 58.5 896-946 bp 1.0 75.2
ycf3-trnS-GGA_p4 ATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.6 58.2 943-993 bp 1.0 75.1
ycf3-trnS-GGA_p5 TTTATCCGCGATCTAGGCAT ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.6 58.2 942-992 bp 1.0 75.1