Hotspot

trnK-UUU-rps16

LSC · 48632-49379 · 748 bp

Back to Camellia

Pi 0.00241
MarkerSeek score 58.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
748
variable_sites
40
pi
0.00241
unique_haplotype_count
49
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 920-958 bp 0.991525 78.1
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 921-959 bp 0.991525 78.0
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 925-963 bp 0.991525 78.0
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 923-961 bp 0.991525 78.0
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC GGTGCTCAACCTACAGAAACT 55.0 47.6 57.9 57.9 932-970 bp 0.940678 75.5