Hotspot

trnH-GUG-psbA

LSC · 44403-44789 · 387 bp

Back to Camellia

Pi 0.0128

MarkerSeek score 54.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 190
pi: 0.0128
unique_haplotype_count: 48
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	496-528 bp	0.991525	85.4
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	497-529 bp	0.991525	85.2
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	497-529 bp	0.991525	84.9
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.2	57.6	498-530 bp	0.991525	84.7
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.7	58.3	489-521 bp	0.991525	83.9