Hotspot

ndhB-trnL-CAA

IRb · 33884-34439 · 556 bp

Back to Camellia

Pi 0

MarkerSeek score 22.20

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRb
length_bp: 556
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 22.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	608-623 bp	1.0	83.5
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	606-621 bp	1.0	83.5
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	50.0	50.0	57.9	58.1	596-611 bp	1.0	82.9
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	603-618 bp	1.0	82.0
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	607-622 bp	1.0	81.4