Hotspot

ycf1

SSC · 123144-128540 · 5397 bp

Back to Camelina

Pi 0.00447

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5397
variable_sites: 65
pi: 0.00447
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAGCCTTCTGAGATGCTTT	CGTTCCCAAAGGCGTAAAAC	50.0	50.0	58.0	58.0	554-560 bp	1.0	82.0
ycf1_p2	CGAGCCTTCTGAGATGCTTT	TTTGCATGGAATCTCTCGGG	50.0	50.0	58.0	58.0	1161-1167 bp	1.0	81.5
ycf1_p3	CGAGCCTTCTGAGATGCTTT	AGTTCCTCGGTGGTCATACA	50.0	50.0	58.0	58.1	810-816 bp	1.0	81.5
ycf1_p4	CGAGCCTTCTGAGATGCTTT	CCGAACCCCAACAAGAAGAA	50.0	50.0	58.0	58.0	955-961 bp	0.857143	75.5
ycf1_p5	CGAGCCTTCTGAGATGCTTT	TTCCGAACCCCAACAAGAAG	50.0	50.0	58.0	58.0	957-963 bp	0.857143	75.5