Hotspot

rpl36-rps8

LSC · 78599-79062 · 464 bp

Back to Camelina

Pi 0.00944

MarkerSeek score 48.60

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 11
pi: 0.00944
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GTTTATGCCTCGGGTTGGAA	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	596-613 bp	1.0	77.0
rpl36-rps8_p2	TTTATGCCTCGGGTTGGAAC	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	595-612 bp	1.0	77.0
rpl36-rps8_p3	GTTTATGCCTCGGGTTGGAA	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	595-612 bp	1.0	76.9
rpl36-rps8_p4	TTTATGCCTCGGGTTGGAAC	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	594-611 bp	1.0	76.9
rpl36-rps8_p5	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.7	58.4	524-541 bp	1.0	76.5