Hotspot

psbM-trnD-GUC

LSC · 27638-28650 · 1013 bp

Back to Camelina

Pi 0.00437
MarkerSeek score 56.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1013
variable_sites
14
pi
0.00437
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACGAGAATGAAGAGTGC TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 1112-1136 bp 1.0 70.5
psbM-trnD-GUC_p2 ACGAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 45.0 50.0 56.9 58.5 1108-1132 bp 1.0 69.8
psbM-trnD-GUC_p3 GGAACGAGAATGAAGAGTGCA TTCAATTGGTCAGAGCACCG 47.6 50.0 58.3 58.5 1111-1135 bp 1.0 68.2
psbM-trnD-GUC_p4 AACGAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 42.9 50.0 57.6 58.5 1109-1133 bp 1.0 67.6
psbM-trnD-GUC_p5 GGAACGAGAATGAAGAGTGC TTCAATTGGTCAGAGCACCG 50.0 50.0 56.5 58.5 1111-1135 bp 1.0 67.1