Hotspot

trnS-GCU-trnG-UCC

LSC · 6707-7346 · 640 bp

Back to Camelina

Pi 0.00342

MarkerSeek score 54.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 7
pi: 0.00342
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	747-755 bp	1.0	73.8
trnS-GCU-trnG-UCC_p2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	745-753 bp	1.0	73.5
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	742-750 bp	1.0	71.8
trnS-GCU-trnG-UCC_p4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	50.0	50.0	57.5	57.2	748-756 bp	1.0	71.5
trnS-GCU-trnG-UCC_p5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	50.0	50.0	57.8	56.9	746-754 bp	1.0	71.4