Hotspot

trnH-GUG-psbA

LSC · 77-354 · 278 bp

Back to Camelina

Pi 0.014

MarkerSeek score 56.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 12
pi: 0.014
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	336-342 bp	1.0	82.3
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	337-343 bp	1.0	82.1
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	351-357 bp	1.0	82.0
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	354-360 bp	1.0	81.9
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	352-358 bp	1.0	81.8