primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	57.9	58.1	0.0	12.9	0.188044	77	354	336-342	1.000000	82.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	58.2	58.1	0.0	12.9	0.213831	77	354	337-343	1.000000	82.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	77	354	351-357	1.000000	82.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	354	354-360	1.000000	81.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	58.2	57.9	0.0	13.4	0.226475	77	354	352-358	1.000000	81.8
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	CTACCGCAGATTTGGACGAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.080684	4325	5465	1263-1836	1.000000	81.3
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4325	5465	1199-1772	1.000000	80.1
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4325	5465	1198-1771	1.000000	80.1
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	CGCAGATTTGGACGAATCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.3	58.1	8.3	16.9	0.419449	4325	5465	1259-1832	1.000000	78.6
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	CTCTACCGCAGATTTGGACG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	0.0	16.9	0.515178	4325	5465	1265-1838	1.000000	77.8
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.8	57.2	0.0	0.0	0.926353	6707	7346	747-755	1.000000	73.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	58.2	57.2	2.0	0.0	0.965511	6707	7346	745-753	1.000000	73.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.6	57.2	0.9	0.0	1.172484	6707	7346	742-750	1.000000	71.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.5	57.2	0.0	0.0	1.213145	6707	7346	748-756	1.000000	71.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	20	20	50.0	50.0	57.8	56.9	0.0	0.0	1.225207	6707	7346	746-754	1.000000	71.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCGGGTATTGTAGATGTTCCC	CCGGATTTGAACTGGGGAAA	21	20	52.4	50.0	57.9	58.1	0.0	0.0	1.203866	25084	26194	841-1214	1.000000	76.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GTTCCCTCTTTTTCATCCCCA	CCGGATTTGAACTGGGGAAA	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.251640	25084	26194	826-1199	1.000000	75.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TGTTCCCTCTTTTTCATCCCC	CCGGATTTGAACTGGGGAAA	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.251640	25084	26194	827-1200	1.000000	75.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCTCTTTTTCATCCCCAAGC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	56.7	58.1	0.0	0.0	1.417112	25084	26194	822-1195	1.000000	74.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGGGTATTGTAGATGTTCCC	AAAAGGATTTGCAGTCCCCC	21	20	52.4	50.0	57.9	58.4	0.0	14.6	1.477229	25084	26194	823-1196	1.000000	73.8
petN-psbM_p1	petN-psbM	1	TCGTAGTGTGGGGAAGAAGT	GCACTCTTCATTCTCGTTCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.039320	26998	27532	636-640	1.000000	72.7
petN-psbM_p2	petN-psbM	2	CGTAGTGTGGGGAAGAAGTG	GCACTCTTCATTCTCGTTCCT	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.114236	26998	27532	635-639	1.000000	72.1
petN-psbM_p3	petN-psbM	3	GGGGCAGAATTCACATGGAT	GCACTCTTCATTCTCGTTCCT	20	21	50.0	47.6	57.9	58.0	8.4	0.0	1.111637	26998	27532	711-715	1.000000	72.0
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	ACTGCACTCTTCATTCTCGT	20	20	50.0	45.0	58.0	56.9	0.0	0.0	1.123080	26998	27532	639-643	1.000000	72.0
petN-psbM_p5	petN-psbM	5	GGGGCAGAATTCACATGGAT	ACTGCACTCTTCATTCTCGT	20	20	50.0	45.0	57.9	56.9	8.4	0.0	1.195397	26998	27532	714-718	1.000000	71.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.0	58.5	0.0	5.4	1.511281	27638	28650	1112-1136	1.000000	70.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	56.9	58.5	0.0	5.4	1.595041	27638	28650	1108-1132	1.000000	69.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GGAACGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.3	58.5	0.0	5.4	1.788824	27638	28650	1111-1135	1.000000	68.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	57.6	58.5	0.0	5.4	1.870420	27638	28650	1109-1133	1.000000	67.6
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.5	58.5	0.0	5.4	1.931209	27638	28650	1111-1135	1.000000	67.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	30209	31237	1178-1187	1.000000	80.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.128554	30209	31237	1181-1190	1.000000	80.5
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.325138	30209	31237	1155-1164	1.000000	78.9
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TGGCCCTTTTAACTCAGTGG	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.328696	30209	31237	1194-1203	1.000000	78.8
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCCCTTTTAACTCAGTGGT	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.330309	30209	31237	1193-1202	1.000000	78.8
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	46058	47372	565-1405	1.000000	85.1
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	46058	47372	562-1402	1.000000	81.9
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.0	58.1	0.0	20.1	1.013432	46058	47372	554-1394	1.000000	81.3
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	20	20	50.0	55.0	57.5	58.6	0.0	0.0	1.064405	46058	47372	527-1367	1.000000	81.0
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.079322	46058	47372	537-1377	1.000000	80.9
atpB-rbcL_p1	atpB-rbcL	1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.0	58.0	9.5	0.0	1.063706	53375	54173	943-945	1.000000	73.0
atpB-rbcL_p2	atpB-rbcL	2	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.1	58.0	7.4	0.0	1.089370	53375	54173	885-887	1.000000	72.9
atpB-rbcL_p3	atpB-rbcL	3	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.0	58.1	9.5	0.0	1.096994	53375	54173	942-944	1.000000	72.7
atpB-rbcL_p4	atpB-rbcL	4	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.1	58.1	7.4	0.0	1.122658	53375	54173	884-886	1.000000	72.6
atpB-rbcL_p5	atpB-rbcL	5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.1	58.0	9.5	0.0	1.143136	53375	54173	941-943	1.000000	72.4
psbE-petL_p1	psbE-petL	1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	57.9	56.3	0.0	12.5	1.783771	63410	64790	1511-1537	1.000000	67.6
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	63410	64790	1429-1455	1.000000	67.0
psbE-petL_p3	psbE-petL	3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.865987	63410	64790	1512-1538	1.000000	66.9
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	20	20	55.0	45.0	58.2	56.3	0.9	12.5	1.909925	63410	64790	1424-1450	1.000000	66.7
psbE-petL_p5	psbE-petL	5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	20	21	45.0	42.9	57.9	57.0	0.0	12.5	2.027428	63410	64790	1511-1537	1.000000	65.6
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	AAACCTGCTAACGAACCGAA	20	20	45.0	45.0	57.9	57.8	5.7	0.0	0.357512	65654	66038	488-494	1.000000	79.8
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	AAAACAAACGCGCTACCAAG	CTGCTAACGAACCGAACCAT	20	20	45.0	50.0	57.9	58.3	5.7	0.0	0.391899	65654	66038	484-490	1.000000	79.5
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CAAAACAAACGCGCTACCAA	AAACCTGCTAACGAACCGAA	20	20	45.0	45.0	58.2	57.8	5.7	0.0	0.414770	65654	66038	489-495	1.000000	79.3
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	TTGTACCCAAAACAAACGCG	AAACCTGCTAACGAACCGAA	20	20	45.0	45.0	57.8	57.8	3.5	0.0	0.433927	65654	66038	496-502	1.000000	79.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CAAAACAAACGCGCTACCAA	CTGCTAACGAACCGAACCAT	20	20	45.0	50.0	58.2	58.3	5.7	0.0	0.449157	65654	66038	485-491	1.000000	79.0
psaJ-rpl33_p1	psaJ-rpl33	1	TTCGGTTCGTTAGCAGGTTT	ATATCTGGAAATTCCCGCCG	20	20	45.0	50.0	57.8	57.8	0.0	9.9	0.428606	66168	66603	603-616	1.000000	79.8
psaJ-rpl33_p2	psaJ-rpl33	2	ATGGTTCGGTTCGTTAGCAG	ATATCTGGAAATTCCCGCCG	20	20	50.0	50.0	58.3	57.8	0.0	9.9	0.462993	66168	66603	607-620	1.000000	79.5
psaJ-rpl33_p3	psaJ-rpl33	3	TTCGGTTCGTTAGCAGGTTT	AATTCCCGCCGATTCTTTCT	20	20	45.0	45.0	57.8	57.6	0.0	0.0	0.663661	66168	66603	594-607	1.000000	78.0
psaJ-rpl33_p4	psaJ-rpl33	4	TTCGGTTCGTTAGCAGGTTT	ATTCCCGCCGATTCTTTCTT	20	20	45.0	45.0	57.8	57.6	0.0	0.0	0.663661	66168	66603	593-606	1.000000	78.0
psaJ-rpl33_p5	psaJ-rpl33	5	GTTCGGTTCGTTAGCAGGTT	ATATCTGGAAATTCCCGCCG	20	20	50.0	50.0	58.5	57.8	0.0	9.9	0.679579	66168	66603	604-617	1.000000	77.8
petD-rpoA_p1	petD-rpoA	1	CCGTCTTTTTGATTGGCACC	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	57.9	55.6	0.0	0.0	4.496545	76695	76900	395-396	1.000000	45.4
petD-rpoA_p2	petD-rpoA	2	CTTTTTGATTGGCACCGTGG	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	58.2	55.6	0.0	0.0	4.649928	76695	76900	391-392	1.000000	44.2
petD-rpoA_p3	petD-rpoA	3	CAGTAGCGACAACCGTCTTT	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	58.2	55.6	0.0	0.0	4.652402	76695	76900	407-408	1.000000	44.2
petD-rpoA_p4	petD-rpoA	4	CCGTCTTTTTGATTGGCACC	TGGACCTTTTGAATAACAGTCAAG	20	24	50.0	37.5	57.9	57.4	0.0	0.0	4.698073	76695	76900	395-396	1.000000	43.8
petD-rpoA_p5	petD-rpoA	5	CGTCTTTTTGATTGGCACCG	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	58.3	55.6	0.0	0.0	4.746670	76695	76900	394-395	1.000000	43.4
rpl36-rps8_p1	rpl36-rps8	1	GTTTATGCCTCGGGTTGGAA	TCGGCGGAGAAATTTTGTGT	20	20	50.0	45.0	58.2	58.4	0.0	7.2	0.570997	78599	79062	596-613	1.000000	77.0
rpl36-rps8_p2	rpl36-rps8	2	TTTATGCCTCGGGTTGGAAC	TCGGCGGAGAAATTTTGTGT	20	20	50.0	45.0	58.2	58.4	0.0	7.2	0.570997	78599	79062	595-612	1.000000	77.0
rpl36-rps8_p3	rpl36-rps8	3	GTTTATGCCTCGGGTTGGAA	CGGCGGAGAAATTTTGTGTT	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.586718	78599	79062	595-612	1.000000	76.9
rpl36-rps8_p4	rpl36-rps8	4	TTTATGCCTCGGGTTGGAAC	CGGCGGAGAAATTTTGTGTT	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.586718	78599	79062	594-611	1.000000	76.9
rpl36-rps8_p5	rpl36-rps8	5	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	20	20	45.0	45.0	57.7	58.4	0.0	7.2	0.659243	78599	79062	524-541	1.000000	76.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTGGACAGCGTTGA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.7	57.9	6.2	0.0	0.449860	112838	113506	799-820	1.000000	78.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGACAGCGTTG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.456340	112838	113506	800-821	1.000000	78.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGATATTGGACAGCGTTGA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	57.7	57.8	6.2	0.0	0.510587	112838	113506	792-813	1.000000	77.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGACAGCGTTG	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	57.7	57.8	0.0	0.0	0.517067	112838	113506	793-814	1.000000	77.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGATATTGGACAGCGTTGA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	57.7	57.8	6.2	0.0	0.521878	112838	113506	797-818	1.000000	77.8
ycf1_p1	ycf1	1	CGAGCCTTCTGAGATGCTTT	CGTTCCCAAAGGCGTAAAAC	20	20	50.0	50.0	58.0	58.0	8.2	0.0	0.062070	126224	126373	554-560	1.000000	82.0
ycf1_p2	ycf1	2	CGAGCCTTCTGAGATGCTTT	TTTGCATGGAATCTCTCGGG	20	20	50.0	50.0	58.0	58.0	8.2	0.0	0.064041	126224	126373	1161-1167	1.000000	81.5
ycf1_p3	ycf1	3	CGAGCCTTCTGAGATGCTTT	AGTTCCTCGGTGGTCATACA	20	20	50.0	50.0	58.0	58.1	8.2	0.0	0.088334	126224	126373	810-816	1.000000	81.5
ycf1_p4	ycf1	4	CGAGCCTTCTGAGATGCTTT	CCGAACCCCAACAAGAAGAA	20	20	50.0	50.0	58.0	58.0	8.2	0.0	0.045695	126224	126373	955-961	0.857143	75.5
ycf1_p5	ycf1	5	CGAGCCTTCTGAGATGCTTT	TTCCGAACCCCAACAAGAAG	20	20	50.0	50.0	58.0	58.0	8.2	0.0	0.045695	126224	126373	957-963	0.857143	75.5