Hotspot

ycf1

SSC · 124310-130321 · 6012 bp

Back to Calystegia

Pi 0.00244

MarkerSeek score 51.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6012
variable_sites: 22
pi: 0.00244
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCGAATGCTTTGACCGATA	TATTGAATCCTGGGTTGCGG	45.0	50.0	58.0	57.9	791-839 bp	1.0	80.7
ycf1_p2	CGATGCAGGAGATAGTCACG	TATTGAATCCTGGGTTGCGG	55.0	50.0	58.0	57.9	860-908 bp	1.0	80.4
ycf1_p3	ACTCGAATCCTCCTTCGGAT	TATTGAATCCTGGGTTGCGG	50.0	50.0	57.9	57.9	913-961 bp	1.0	80.1
ycf1_p4	TGCGAATGCTTTGACCGATA	CCAGAATTTGTGCGGCTTTT	45.0	45.0	58.0	57.8	463-511 bp	1.0	80.1
ycf1_p5	TCCTCCTTCGGATTCCACAT	TATTGAATCCTGGGTTGCGG	50.0	50.0	58.1	57.9	906-954 bp	1.0	79.7