Hotspot

rps16

LSC · 4740-5800 · 1061 bp

Back to Calystegia

Pi 0.00251

MarkerSeek score 54.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1061
variable_sites: 4
pi: 0.00251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTTTTCCTTAATTGAATTTGGC	TCAAGTCCATACCGGTTCCA	27.3	50.0	52.1	58.4	1123-1124 bp	1.0	40.4
rps16_p2	TTTTTCCTTAATTGAATTTGGC	TGCCTACACATCAACCGAAC	27.3	50.0	52.1	58.2	1184-1185 bp	1.0	40.3
rps16_p3	TTTTTCCTTAATTGAATTTGGC	CCTACACATCAACCGAACCC	27.3	55.0	52.1	58.3	1182-1183 bp	1.0	40.3
rps16_p4	TTTTTCCTTAATTGAATTTGGC	GATGCCTACACATCAACCGA	27.3	50.0	52.1	57.7	1186-1187 bp	1.0	40.3
rps16_p5	TTTTTCCTTAATTGAATTTGGC	CTACACATCAACCGAACCCA	27.3	50.0	52.1	57.5	1181-1182 bp	1.0	40.3