Hotspot

psbC

LSC · 35264-36685 · 1422 bp

Back to Calystegia

Pi 0

MarkerSeek score 36.50

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCAGCGGAAGATCCTGAATT	GCATTGGATCTCTCGTCTCA	50.0	50.0	58.3	57.1	1538 bp	1.0	70.4
psbC_p2	AGGTATTCGTGCTTGGATGG	GCATTGGATCTCTCGTCTCA	50.0	50.0	57.7	57.1	1482 bp	1.0	70.3
psbC_p3	GCAGCGGAAGATCCTGAATT	AGCGATTCCTACTTCAAGCA	50.0	45.0	58.3	56.9	1555 bp	1.0	68.9
psbC_p4	AGGTATTCGTGCTTGGATGG	AGCGATTCCTACTTCAAGCA	50.0	45.0	57.7	56.9	1499 bp	1.0	68.9
psbC_p5	TCTCAAGAAATTCGGGCAGC	GCATTGGATCTCTCGTCTCA	50.0	50.0	58.5	57.1	1553 bp	1.0	68.6