Hotspot

ndhA

SSC · 119794-122353 · 2560 bp

Back to Calystegia

Pi 0.00182

MarkerSeek score 56.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2560
variable_sites: 7
pi: 0.00182
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGAGGCAAGCC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.4	57.9	2626-2638 bp	1.0	76.1
ndhA_p2	TCTCTTGTTTGAGGCAAGCC	GGGGGAAGTTGATCGTTGAG	50.0	55.0	58.4	58.3	2624-2636 bp	1.0	75.0
ndhA_p3	TCTCTTGTTTGAGGCAAGCC	CCCACCGGGTTTGATCAATT	50.0	50.0	58.4	58.4	2723-2735 bp	1.0	73.5
ndhA_p4	GCAAGCCGTCAATCTTGAAT	ATGGGGGAAGTTGATCGTTG	45.0	50.0	57.1	57.9	2613-2625 bp	1.0	71.8
ndhA_p5	TCTCTTGTTTGAGGCAAGCC	CCGGGTTTGATCAATTTGCA	50.0	45.0	58.4	57.3	2719-2731 bp	1.0	71.2