Hotspot

psaC-ndhE

SSC · 117523-117786 · 264 bp

Back to Calystegia

Pi 0.00505

MarkerSeek score 55.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 264
variable_sites: 2
pi: 0.00505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGCTCGGACACATTGAGTAC	GCTATTGGGCTGGCTATTGT	55.0	50.0	58.4	58.0	408-409 bp	1.0	77.4
psaC-ndhE_p2	GCTCGGACACATTGAGTACA	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.6	58.0	407-408 bp	1.0	77.4
psaC-ndhE_p3	AGGCTCGGACACATTGAGTA	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.4	58.0	409-410 bp	1.0	76.7
psaC-ndhE_p4	TTTAGCCTTACAACCGTCCC	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.5	58.0	459-460 bp	1.0	76.4
psaC-ndhE_p5	CGTCCCACGGTATCATTTCT	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.4	58.0	445-446 bp	1.0	75.5