Hotspot

rpl23-trnI-CAU

IRb · 87812-88105 · 294 bp

Back to Calystegia

Pi 0.0159

MarkerSeek score 43.40

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: IRb
length_bp: 294
variable_sites: 7
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	TGATTTTTCCCCAATAACCGA	GGCGAAGTCGTAGGTTCAAT	38.1	50.0	55.5	58.0	390 bp	1.0	53.1
rpl23-trnI-CAU_p2	TGATTTTTCCCCAATAACCGA	ATTGGCGAAGTCGTAGGTTC	38.1	50.0	55.5	58.0	393 bp	1.0	53.0
rpl23-trnI-CAU_p3	TGATTTTTCCCCAATAACCGA	CCTGTTAAGCATCCATGGCT	38.1	50.0	55.5	57.9	438 bp	1.0	52.4
rpl23-trnI-CAU_p4	TGATTTTTCCCCAATAACCGA	GACAAGGGTCCCTGTTAAGC	38.1	55.0	55.5	58.2	448 bp	1.0	51.5
rpl23-trnI-CAU_p5	TGATTTTTCCCCAATAACCGA	TGAAAGCGCCCAACTCATAA	38.1	45.0	55.5	57.8	412 bp	1.0	51.5