Hotspot

rbcL-psaI

LSC · 58316-61829 · 3514 bp

Back to Calystegia

Pi 0.00247

MarkerSeek score 30.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 3514
variable_sites: 13
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGGAATTCTAGCTATCTGGCT	ACTCTCGCCTTCGCTACTAT	42.9	50.0	55.9	58.0	237 bp	1.0	56.1
rbcL-psaI_p2	TGGAATTCTAGCTATCTGGCT	TTTCACCACCACATACGGAC	42.9	50.0	55.9	57.8	211 bp	1.0	55.1
rbcL-psaI_p3	TGGAATTCTAGCTATCTGGCT	ACACCCTTCATGTGAGTTCG	42.9	50.0	55.9	57.8	266 bp	1.0	54.9
rbcL-psaI_p4	TGGAATTCTAGCTATCTGGCT	CACCCTTCATGTGAGTTCGT	42.9	50.0	55.9	57.8	265 bp	1.0	54.9
rbcL-psaI_p5	TGGAATTCTAGCTATCTGGCT	TCTACTGGAACTCTCGCCTT	42.9	50.0	55.9	57.8	246 bp	1.0	54.6