Hotspot

psaA-ycf3

LSC · 43316-44072 · 757 bp

Back to Calystegia

Pi 0.00528

MarkerSeek score 51.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 6
pi: 0.00528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGATGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	924-935 bp	1.0	80.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGATGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.0	58.0	906-917 bp	1.0	80.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGATGT	AGACTTGGTTCGATCAAGCC	50.0	50.0	58.0	57.9	936-947 bp	1.0	80.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGATGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.0	57.9	858-869 bp	1.0	80.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGATGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.0	58.1	926-937 bp	1.0	80.0