Hotspot

psbC-trnS-UGA

LSC · 36686-36925 · 240 bp

Back to Calystegia

Pi 0.0278

MarkerSeek score 57.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 10
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	359 bp	1.0	80.8
psbC-trnS-UGA_p2	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	439 bp	1.0	80.1
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	339 bp	1.0	80.1
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	TCTTTCGGAGAGATGGCTGA	55.0	50.0	58.2	58.1	366 bp	1.0	79.7
psbC-trnS-UGA_p5	GCAGCCGGATTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	398 bp	1.0	79.5