Hotspot

psbM-trnD-GUC

LSC · 31020-31684 · 665 bp

Back to Calystegia

Pi 0.00201

MarkerSeek score 60.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 2
pi: 0.00201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAGGAGAAAGGCGGTAGGAA	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.3	58.5	798-799 bp	1.0	73.6
psbM-trnD-GUC_p2	TAAGGAGAAAGGCGGTAGGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.5	58.5	799-800 bp	1.0	72.1
psbM-trnD-GUC_p3	AAGGAGAAAGGCGGTAGGAA	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.3	57.0	799-800 bp	1.0	69.4
psbM-trnD-GUC_p4	ATAAGGAGAAAGGCGGTAGGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	800-801 bp	1.0	68.0
psbM-trnD-GUC_p5	TAAGGAGAAAGGCGGTAGGA	GTTCAATTGGTCAGAGCACC	50.0	50.0	57.5	57.0	800-801 bp	1.0	67.9